CD18 Antibody [M18/2]

Product Specifications

Background

The M18/2 monoclonal antibody specifically binds to mouse CD18, a 95kDA integrin beta 2 glycoprotein. CD18 is expressed on all leukocytes with the highest density by NK and T cells. CD18 forms LFA-1 with CD11a, Mac-1 with CD11b and gp150/95 with CD11c. Through these interactions, CD18 has an important role in cellular adhesion. The M18/2 antibody is reported to stimulate Mac-1 and LFA-1 adhesion to their ligands and block tumor cell metastasis.

NCBI Gene ID

16414

Swiss Prot

P11835

Host

Rat

Reactivity

Mouse

Clonality

Monoclonal

Clone

M18/2

Conjugation

Unconjugated

Type

Primary Antibodies, Functional Antibodies

Field of Research

Cancer

Purification

The monoclonal antibody was purified utilizing affinity chromatography. The endotoxin level is determined by LAL test to be less than 0.01 EU/µg of the protein.

Concentration

Batch dependent

Buffer

Phosphate-buffered aqueous solution, ph7.2.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

The product should be stored undiluted at 4˚ C . Do not freeze.

Fragment

Rat IgG2a, kappa

Specificity

The M18/2 monoclonal antibody specifically binds to mouse CD18, a 95kDA integrin beta 2 glycoprotein.

Symbol

Itgb2

NCBI Official Name

Integrin beta 2

NCBI Organism

Mus musculus

Background Reference 01

Schlossman, S., L. Bloumsell, et al. eds (1995) . Leucocyte Typing V: White Cell Differentiation Antigens. Oxford University Press. New York

Background Reference 02

Nuns, J., Klasen, S., Ragueneau, M., Pavon, C., Couez, D., Mawas, C., ... Olive, D. (1993) . CD28 mAbs with distinct binding properties differ in their ability to induce T cell activation: analysis of early and late activation events.International immunology,5 (3), 311-315.

Background Reference 03

Karlsson, I., Malleret, B., Brochard, P., Delache, B., Calvo, J., Le Grand, R., Vaslin, B. (2007) . FoxP3+ CD25+ CD8+ T-cell induction during primary simian immunodeficiency virus infection in cynomolgus macaques correlates with low CD4+ T-cell activation and high viral load.Journal of virology,81 (24), 13444-13455.