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CD8a Antibody [RPA-T8] (FITC)

Product Specifications

Background

The RPA-T8 monoclonal antibody specifically reacts with the human CD8a molecule, a 32 kDa cell surface receptor expressed either as a heterodimer (CD8 alpha/beta) or as a homodimer (CD8 alpha/alpha) on the majority of thymocytes, a subpopulation of mature T cells, and natural killer cells. CD8 interacts with the major histocompatibility complex class I (MHC class I) molecules on antigen-presenting cells or epithelial cells. The RPA-T8 antibody reacts with 13-48% of peripheral lymphocytes, 80% of thymocytes, and a subset of natural killer cells. RPA-T8, OKT8, and HIT8a antibodies do not compete with each other for binding to peripheral leukocytes, meaning that that they do not recognize the same epitope or block each other by steric hindrance.

NCBI Gene ID

925

Swiss Prot

P01732

Host

Mouse

Reactivity

Human

Clonality

Monoclonal

Clone

RPA-T8

Conjugation

FITC

Type

Primary Antibodies

Field of Research

Immunology

Purification

The monoclonal antibody was purified utilizing affinity chromatography and unreacted dye was removed from the product.

Concentration

Batch dependent

Buffer

Phosphate-buffered aqueous solution, ≤0.09% Sodium azide, may contain carrier protein/stabilizer, ph7.2.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

The product should be stored undiluted at 4˚ C and should be protected from prolonged exposure to light. Do not freeze.

Fragment

Mouse IgG1, kappa

Specificity

The RPA-T8 monoclonal antibody specifically reacts with the human CD8a molecule, a 32 kDa cell surface receptor expressed either as a heterodimer (CD8 alpha/beta) or as a homodimer (CD8 alpha/alpha) on the majority of thymocytes, a subpopulation of mature T cells, and natural killer cells.

Symbol

CD8A

NCBI Official Name

CD8a molecule

NCBI Organism

Homo sapiens

Background Reference 01

Knapp W (1989) Leucocyte typing IV: white cell differentiation antigens. Oxford University Press, 1989.

Background Reference 02

Schlossman, S., L. Bloumsell, et al. eds (1995) . Leucocyte Typing V: White Cell Differentiation Antigens. Oxford University Press. New York

Other Product Names

CD8, MAL, p32, Leu2, CD8A

Tested Applications

Flow

Physical Properties

Liquid

Frequently Asked Questions

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