CD8a Antibody [2.43] (APC)

Product Specifications

Background

The 2.43 monoclonal antibody specifically reacts with mouse CD8 antigen. CD8a (the alpha chains) form heterodimers with CD8b (the beta chains) or homodimers (alpha-alpha), which occur as receptors on the surface of the majority of thymocytes. A subpopulation of mature T lymphocytes expresses the CD8 alpha beta (alpha beta TCR T cells), and a subpopulation of intestinal intraepithelial lymphocytes and dendritic cells express CD8a without CD8b. CD8 interacts with the mouse major histocompatibility complex class I (MHC class I) molecules on antigen-presenting cells or epithelial cells.

NCBI Gene ID

12525

Swiss Prot

P01731

Host

Rat

Reactivity

Mouse

Clonality

Monoclonal

Clone

2.43

Conjugation

APC

Type

Primary Antibodies

Field of Research

Immunology

Purification

The monoclonal antibody was purified utilizing affinity chromatography and unreacted dye was removed from the product.

Concentration

Batch dependent

Buffer

Phosphate-buffered aqueous solution, ≤0.09% Sodium azide, may contain carrier protein/stabilizer, ph7.2.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

The product should be stored undiluted at 4˚ C and should be protected from prolonged exposure to light. Do not freeze.

Fragment

Rat IgG2b

Specificity

The 2.43 monoclonal antibody specifically reacts with mouse CD8 antigen.

Symbol

Cd8a

NCBI Official Name

CD8 antigen, alpha chain

NCBI Organism

Mus musculus

Background Reference 01

Sayos, J., Wu, C., Morra, M., Wang, N., Zhang, X., Allen, D., ... Terhorst, C. (1998) . The X-linked lymphoproliferative-disease gene product SAP regulates signals induced through the co-receptor SLAM.Nature,395 (6701), 462-469.

Background Reference 02

Thomas, Y., Sosman, J., Irigoyen, O., Friedman, S. M., Kung, P. C., Goldstein, G., Chess, L. (1980) . Functional analysis of human T cell subsets defined by monoclonal antibodies. I. Collaborative TT interactions in the immunoregulation of B cell differentiation.The Journal of Immunology,125 (6), 2402-2408.

Background Reference 03

Campanelli, R., Palermo, B., Garbelli, S., Mantovani, S., Lucchi, P., Necker, A., ... Giachino, C. (2002) . Human CD8 coreceptor is strictly involved in MHCpeptide tetramerTCR binding and T cell activation.International immunology,14 (1), 39-44.