CD86 Antibody [GL-1 (GL1) ] (APC)

Product Specifications

Background

The GL-1 monoclonal antibody reacts specifically with the mouse B7-2 (also known as CD86), a costimulatory molecule expressed by B and T lymphocytes, macrophages (thioglycollate-induced from peritoneum), astrocytes and dendritic cells. The memory CD4+ T lymphocytes express CD86 (as mRNA and protein) . CD86, and the B7-1 (CD 80) molecule, are ligands for CD152 and CD28, and influence the costimulatory interactions between lymphocytes B and T. B7-2 is also involved in the mouse natural killer cell-mediated cytotoxicity. The GL-1 antibody blocks the mixed lymphocyte reaction (MLR) and inhibits the T-cells stimulation by antigen-presenting cells. Mixtures of anti-B7-1 antibody and GL-1 were reported to inhibit the interaction between CD125 and its ligand with the in vivo priming of cytotoxic T lymphocytes.

NCBI Gene ID

12524

Swiss Prot

P42082

Host

Rat

Reactivity

Mouse

Clonality

Monoclonal

Clone

GL-1 (GL1)

Conjugation

APC

Type

Primary Antibodies

Field of Research

Innate Immunity

Purification

The monoclonal antibody was purified utilizing affinity chromatography and unreacted dye was removed from the product.

Concentration

Batch dependent

Buffer

Phosphate-buffered aqueous solution, ≤0.09% Sodium azide, may contain carrier protein/stabilizer, ph7.2.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

The product should be stored undiluted at 4˚ C and should be protected from prolonged exposure to light. Do not freeze.

Fragment

Rat IgG2a, kappa

Specificity

Symbol

Cd86

NCBI Official Name

CD86 antigen

NCBI Organism

Mus musculus

Background Reference 01

Maeda, K., Sato, T., Azuma, M., Yagita, H., Okumura, K. (1997) . Characterization of rat CD80 and CD86 by molecular cloning and mAb.International immunology,9 (7), 993-1000.