What is the catalog number for CD86 Antibody [GL-1 (GL1) ] (PE)?

The catalog number for CD86 Antibody [GL-1 (GL1) ] (PE) is 76-602.

What sizes are available for CD86 Antibody [GL-1 (GL1) ] (PE)?

CD86 Antibody [GL-1 (GL1) ] (PE) is available in the following size(s): 0.1 mg.

Is CD86 Antibody [GL-1 (GL1) ] (PE) in stock?

CD86 Antibody [GL-1 (GL1) ] (PE) is currently made to order / not held in immediate stock. Contact us for lead time.

What is the recommended positive control for this assay?

A recommended positive control, where one is specified by the manufacturer, is listed on the datasheet or in the assay protocol.

How should this product be stored?

Storage conditions depend on the specific reagent and are listed on the product datasheet, with most antibodies and proteins requiring -20°C unless stated otherwise. It is your responsibility to store the product correctly immediately upon receipt; damage from improper storage after delivery is not eligible for replacement.

Is this product animal-origin-free?

Animal-origin-free status, where applicable, is noted on the datasheet under formulation details.

CD86 Antibody [GL-1 (GL1) ] (PE)

Product Specifications

Background

The GL-1 monoclonal antibody reacts specifically with the mouse B7-2 (also known as CD86), a costimulatory molecule expressed by B and T lymphocytes, macrophages (thioglycollate-induced from peritoneum), astrocytes and dendritic cells. The memory CD4+ T lymphocytes express CD86 (as mRNA and protein) . CD86, and the B7-1 (CD 80) molecule, are ligands for CD152 and CD28, and influence the costimulatory interactions between lymphocytes B and T. B7-2 is also involved in the mouse natural killer cell-mediated cytotoxicity. The GL-1 antibody blocks the mixed lymphocyte reaction (MLR) and inhibits the T-cells stimulation by antigen-presenting cells. Mixtures of anti-B7-1 antibody and GL-1 were reported to inhibit the interaction between CD125 and its ligand with the in vivo priming of cytotoxic T lymphocytes.

NCBI Gene ID

12524

Swiss Prot

P42082

Host

Rat

Reactivity

Mouse

Clonality

Monoclonal

Clone

GL-1 (GL1)

Conjugation

Type

Primary Antibodies

Field of Research

Innate Immunity

Purification

The monoclonal antibody was purified utilizing affinity chromatography and unreacted dye was removed from the product.

Concentration

Batch dependent

Buffer

Phosphate-buffered aqueous solution, ≤0.09% Sodium azide, may contain carrier protein/stabilizer, ph7.2.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

The product should be stored undiluted at 4˚ C and should be protected from prolonged exposure to light. Do not freeze.

Fragment

Rat IgG2a, kappa

Specificity

Symbol

Cd86

NCBI Official Name

CD86 antigen

NCBI Organism

Mus musculus

Background Reference 01

Hathcock, K. S., Laszlo, G., Dickler, H. B., Bradshaw, J., Linsley, P., Hodes, R. J. (1993) . Identification of an alternative CTLA-4 ligand costimulatory for T cell activation.Science,262 (5135), 905-907.

Background Reference 02

Inaba, K., Witmer-Pack, M., Inaba, M., Hathcock, K. S., Sakuta, H., Azuma, M., ... Steinman, R. M. (1994) . The tissue distribution of the B7-2 costimulator in mice: abundant expression on dendritic cells in situ and during maturation in vitro.The Journal of experimental medicine,180 (5), 1849-1860.

Background Reference 03

Hathcock, K. S., Laszlo, G., Pucillo, C., Linsley, P., Hodes, R. J. (1994) . Comparative analysis of B7-1 and B7-2 costimulatory ligands: expression and function.The Journal of experimental medicine,180 (2), 631-640.