CD16/CD32 Antibody [2.4G2]

The 2.4G2 monoclonal antibody specifically reacts with an epitope on the extracellular domain of the mouse CD16 (Fc gamma III) and CD 32 (Fc gamma II) . CD16 and CD32 are low affinity receptors for the IgG Fc domain and are expressed by B lymphocytes, NK cells, kupffer cells, mast cells, monocytes, macrophages, granulocytes, immature thymocytes, neutrophils, and some activated mature T cells.The 2.4G2 antibody blocks the binding of immunoglobulins to CD16 and CD32, and possibly to Fc gamma I receptor.

The monoclonal antibody was purified utilizing affinity chromatography. The endotoxin level is determined by LAL test to be less than 0.01 EU/µg of the protein.

The 2.4G2 monoclonal antibody specifically reacts with an epitope on the extracellular domain of the mouse CD16 (Fc gamma III) and CD 32 (Fc gamma II) .

Araujo-Jorge, T. A. N. I. A., Rivera, M. T., el Bouhdidi, A. Y. A. C. H. I., Daron, M. A. R. C., Carlier, Y. (1993) . An Fc gamma RII-, Fc gamma RIII-specific monoclonal antibody (2.4 G2) decreases acute Trypanosoma cruzi infection in mice.Infection and immunity,61 (11), 4925-4928.

Jensen, W. A., Marschner, S., Ott, V. L., Cambier, J. C. (2001) . FcgammaRIIB-mediated inhibition of T-cell receptor signal transduction involves the phosphorylation of SH2-containing inositol 5-phosphatase (SHIP), dephosphorylation of the linker of activated T-cells (LAT) and inhibition of calcium mobilization.Biochemical Society Transactions,29 (Pt 6), 840-846.

Vremec, D., Zorbas, M., Scollay, R., Saunders, D. J., Ardavin, C. F., Wu, L., Shortman, K. (1992) . The surface phenotype of dendritic cells purified from mouse thymus and spleen: investigation of the CD8 expression by a subpopulation of dendritic cells.The Journal of experimental medicine,176 (1), 47-58.