CD16 Antibody [2.4G2]

Product Specifications

Background

The 2.4G2 monoclonal antibody specifically reacts with an epitope on the extracellular domain of the mouse CD16 (Fc gamma III) and CD 32 (Fc gamma II) . CD16 and CD32 are low affinity receptors for the IgG Fc domain and are expressed by B lymphocytes, NK cells, kupffer cells, mast cells, monocytes, macrophages, granulocytes, immature thymocytes, neutrophils, and some activated mature T cells.The 2.4G2 antibody blocks the binding of immunoglobulins to CD16 and CD32, and possibly to Fc gamma I receptor.

NCBI Gene ID

14131

Swiss Prot

P08508

Host

Rat

Reactivity

Mouse

Clonality

Monoclonal

Clone

2.4G2

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Innate Immunity

Purification

The monoclonal antibody was purified utilizing affinity chromatography and unreacted dye was removed from the product.

Concentration

Batch dependent

Buffer

Phosphate-buffered aqueous solution, ≤0.09% Sodium azide, may contain carrier protein/stabilizer, ph7.2.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

The product should be stored undiluted at 4˚ C and should be protected from prolonged exposure to light. Do not freeze.

Fragment

Rat IgG2b

Specificity

The 2.4G2 monoclonal antibody specifically reacts with an epitope on the extracellular domain of the mouse CD16 (Fc gamma III) and CD 32 (Fc gamma II) .

Symbol

Fcgr3

NCBI Official Name

Fc receptor, IgG, low affinity III

NCBI Organism

Mus musculus

Background Reference 01

Araujo-Jorge, T. A. N. I. A., Rivera, M. T., el Bouhdidi, A. Y. A. C. H. I., Daron, M. A. R. C., Carlier, Y. (1993) . An Fc gamma RII-, Fc gamma RIII-specific monoclonal antibody (2.4 G2) decreases acute Trypanosoma cruzi infection in mice.Infection and immunity,61 (11), 4925-4928.

Background Reference 02

Jensen, W. A., Marschner, S., Ott, V. L., Cambier, J. C. (2001) . FcgammaRIIB-mediated inhibition of T-cell receptor signal transduction involves the phosphorylation of SH2-containing inositol 5-phosphatase (SHIP), dephosphorylation of the linker of activated T-cells (LAT) and inhibition of calcium mobilization.Biochemical Society Transactions,29 (Pt 6), 840-846.

Background Reference 03

Vremec, D., Zorbas, M., Scollay, R., Saunders, D. J., Ardavin, C. F., Wu, L., Shortman, K. (1992) . The surface phenotype of dendritic cells purified from mouse thymus and spleen: investigation of the CD8 expression by a subpopulation of dendritic cells.The Journal of experimental medicine,176 (1), 47-58.