CD16 Antibody [CB16]

Product Specifications

Background

The CB15 monoclonal antibody specifically binds to human CD16, a low affinity Fc receptor. It is expressed on NK, neutrophils, macrophages, monocytes, and placental trophoblasts. CD16 is expressed in CD16a and CD16b forms. CD16a is a polyeptide anchored transmembrane protein and CD16b is a monomeric glycosylphosphatidylinositol (GPI) -anchored protein found in neutrophils. CD16 is reported to be involved in NK cell activation and signal transduction.

NCBI Gene ID

2214

Swiss Prot

P08637

Host

Mouse

Reactivity

Human

Clonality

Monoclonal

Clone

CB16

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Innate Immunity

Purification

The monoclonal antibody was purified utilizing affinity chromatography. The endotoxin level is determined by LAL test to be less than 0.01 EU/µg of the protein.

Concentration

Batch dependent

Buffer

Phosphate-buffered aqueous solution, ph7.2.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

The product should be stored undiluted at 4˚ C . Do not freeze.

Fragment

Mouse IgG1

Specificity

The CB15 monoclonal antibody specifically binds to human CD16, a low affinity Fc receptor.

Symbol

FCGR3A

NCBI Official Name

Fc fragment of IgG, low affinity IIIa, receptor (CD16a)

NCBI Organism

Homo sapiens

Background Reference 01

Deaglio, S., Zubiaur, M., Gregorini, A., Bottarel, F., Ausiello, C. M., Dianzani, U., ... Malavasi, F. (2002) . Human CD38 and CD16 are functionally dependent and physically associated in natural killer cells.Blood,99 (7), 2490-2498.

Background Reference 02

Peruzzi, G., Femnou, L., Gil-Krzewska, A., Borrego, F., Weck, J., Krzewski, K., Coligan, J. E. (2013) . Membrane-type 6 matrix metalloproteinase regulates the activation-induced downmodulation of CD16 in human primary NK cells.The Journal of Immunology,191 (4), 1883-1894.

Background Reference 03

Wirthmueller, U., Kurosaki, T., Murakami, M. S., Ravetch, J. V. (1992) . Signal transduction by Fc gamma RIII (CD16) is mediated through the gamma chain.The Journal of experimental medicine,175 (5), 1381-1390.