CD45.2 Antibody [104] (APC)

Product Specifications

Background

The 104 monoclonal antibody specifically reacts with the mouse CD45 molecule, the Leukocyte Common Antigen (LCA) which occurs in the alloantigen CD45.2-expressing mouse strains (C57BL/6, CBA, 129, A, AKR, C58, DBA/1, DBA/2, BALB/c, and C3H/He) . The 104 monoclonal antibody does not react with the leukocytes of the CD45.1-expressing mouse strains (DA, SJL/J, RIII, and STS/A) . The CD45 molecule is a member of the Protein Tyrosine Phosphatase (PTP) family, because its intracellular region contains two PTP domains. The extracellular region’s variability is caused by different levels of glycosylation, and the splicing of the 4, 5, and 6 exons. The isoforms found in the mouse strains depend on the activation state, maturation stage and cell type, and are very important in B and T lymphocytes antigen receptor signal transduction. The 104 antibody inhibits some of the B lymphocytes responses, reduces the serum IgG levels, and influences the autoimmune renal pathology.

NCBI Gene ID

19264

Swiss Prot

P06800

Host

Mouse

Reactivity

Mouse

Clonality

Monoclonal

Clone

104

Conjugation

APC

Type

Primary Antibodies

Field of Research

Immunology

Purification

The monoclonal antibody was purified utilizing affinity chromatography and unreacted dye was removed from the product.

Concentration

Batch dependent

Buffer

Phosphate-buffered aqueous solution, ≤0.09% Sodium azide, may contain carrier protein/stabilizer, ph7.2.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

The product should be stored undiluted at 4˚ C and should be protected from prolonged exposure to light. Do not freeze.

Fragment

Mouse IgG2a, kappa

Specificity

Symbol

Ptprc

NCBI Official Name

Protein tyrosine phosphatase, receptor type, C

NCBI Organism

Mus musculus

Background Reference 01

Shen, F. W., Tung, J. S., Boyse, E. A. (1986) . Further definition of the Ly-5 system.Immunogenetics,24 (3), 146-149.

Background Reference 02

Shen, F.W. (1981) Monoclonal antibodies to mouse lymphocyte differentiation alloantigens. Monoclonal Antibodies and T-Cell Hybridomas: Perspectives and Technical Advances. G.J. H U. H and J.F. Kearney, editors. Elsevier/North-Holland, Amsterdam. pp. 2531.

Background Reference 03

Yakura, H., Shen, F. W., Bourcet, E., Boyse, E. A. (1983) . On the function of Ly-5 in the regulation of antigen-driven B cell differentiation. Comparison and contrast with Lyb-2.The Journal of experimental medicine,157 (4), 1077-1088.

Background Reference 04

Suzuki, K., Oida, T., Hamada, H., Hitotsumatsu, O., Watanabe, M., Hibi, T., ... Ishikawa, H. (2000) . Gut cryptopatches: direct evidence of extrathymic anatomical sites for intestinal T lymphopoiesis.Immunity,13 (5), 691-702.