CD45 Antibody [30-F11] (APC)

Product Specifications

Background

The 30-F11 monoclonal antibody specifically reacts with all isoforms of CD45 and also with the alloantigens CD45.1 and CD45.2 (LCA) . CD45 is a transmembrane glycoprotein, expressed by all the hematopoietic cells, except for platelets and mature erythrocytes, which distinguishes the leukocytes from the non-hematopoietic cells. The CD45 molecule is a member of the Protein Tyrosine Phosphatase (PTP) family, because its intracellular region contains two PTP domains. The extracellular region’s variability is caused by different levels of glycosylation, and the splicing of the 4, 5, and 6 exons.The isoforms found in the mouse strains depend on the activation state, maturation stage and cell type, and are very important in B and T lymphocytes antigen receptor signal transduction.

NCBI Gene ID

19264

Swiss Prot

P06800

Host

Rat

Reactivity

Mouse

Clonality

Monoclonal

Clone

30-F11

Conjugation

APC

Type

Primary Antibodies

Field of Research

Immunology

Purification

The monoclonal antibody was purified utilizing affinity chromatography and unreacted dye was removed from the product.

Concentration

Batch dependent

Buffer

Phosphate-buffered aqueous solution, ≤0.09% Sodium azide, may contain carrier protein/stabilizer, ph7.2.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

The product should be stored undiluted at 4˚ C and should be protected from prolonged exposure to light. Do not freeze.

Fragment

Rat IgG2b, kappa

Specificity

The 30-F11 monoclonal antibody specifically reacts with all isoforms of CD45 and also with the alloantigens CD45.1 and CD45.2 (LCA) .

Symbol

Ptprc

NCBI Official Name

Protein tyrosine phosphatase, receptor type, C

NCBI Organism

Mus musculus

Background Reference 01

Shen, F.W. (1981) Monoclonal antibodies to mouse lymphocyte differentiation alloantigens. Monoclonal Antibodies and T-Cell Hybridomas: Perspectives and Technical Advances. G.J. H U. H and J.F. Kearney, editors. Elsevier/North-Holland, Amsterdam. pp. 2531.

Background Reference 02

Yakura, H. I. D. E. T. A. K. A., Kawabata, I., Shen, F. W., Katagiri, M. (1986) . Selective inhibition of lipopolysaccharide-induced polyclonal IgG response by monoclonal Ly-5 antibody.The Journal of Immunology,136 (8), 2729-2733.

Background Reference 03

Yakura, H., Shen, F. W., Bourcet, E., Boyse, E. A. (1983) . On the function of Ly-5 in the regulation of antigen-driven B cell differentiation. Comparison and contrast with Lyb-2.The Journal of experimental medicine,157 (4), 1077-1088.