What is the catalog number for CD25 Antibody [PC61.5] (Violet-450)?

The catalog number for CD25 Antibody [PC61.5] (Violet-450) is 76-510.

What sizes are available for CD25 Antibody [PC61.5] (Violet-450)?

CD25 Antibody [PC61.5] (Violet-450) is available in the following size(s): 0.025 mg.

Is CD25 Antibody [PC61.5] (Violet-450) in stock?

CD25 Antibody [PC61.5] (Violet-450) is currently made to order / not held in immediate stock. Contact us for lead time.

Is antigen retrieval required for IHC with this antibody?

Recommended antigen retrieval methods, where applicable, are listed on the datasheet under the IHC protocol section.

What temperature does this product ship at?

Shipping temperature is chosen based on the product’s stability needs, ranging from ambient to dry ice, and is indicated on the product page. Any related shipping fee is shown clearly in your quotation.

What is the molecular weight of the target protein?

Molecular weight information, where applicable, is listed on the datasheet under target details, useful for confirming the correct band on western blot.

CD25 Antibody [PC61.5] (Violet-450)

Product Specifications

Background

The PC61.5 antibody specifically reacts with mouse CD25, the 55 kDa low-affinity Interleukin-2 Receptor alpha chain (IL-2R alpha), expressed on early progenitors of T and B lineage, and on B and T cells. Together with CD122 (IL-2 Receptor beta) and CD 132 (IL-2 Receptor gammac, the common gamma chain), CD25 forms high-affinity receptor complexes for IL-2. Resting B and T cells and natural killer cells do not express IL-2Ralpha. Cd25 is also expressed on the dendritic cells, and it enhances lymphocyte differentiation and activation.The PC61.5 antibody block the binding of IL-2 to both high-affinity and low-affinity receptors.BG Violet 450 conjugate is an alternative to the Pacific Blue, eFluor 450, or BD Horizon V450 dyes. It is excited by the violet (405 nm) laser, with a peak emission of 450nm.

NCBI Gene ID

16184

Swiss Prot

P01590

Host

Rat

Reactivity

Mouse

Clonality

Monoclonal

Clone

PC61.5

Conjugation

Violet-450

Type

Primary Antibodies

Field of Research

Immunology

Purification

The monoclonal antibody was purified utilizing affinity chromatography and unreacted dye was removed from the product.

Concentration

Batch dependent

Buffer

Phosphate-buffered aqueous solution, ≤0.09% Sodium azide, may contain carrier protein/stabilizer, ph7.2.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

The product should be stored undiluted at 4˚ C and should be protected from prolonged exposure to light. Do not freeze.

Fragment

Rat IgG1, lambda

Specificity

Symbol

Il2ra

NCBI Official Name

Interleukin 2 receptor, alpha chain

NCBI Organism

Mus musculus

Background Reference 01

Hayashi, T., Hasegawa, K., Adachi, C. (2005) . Elimination of CD4+ CD25+ T cell accelerates the development of glomerulonephritis during the preactive phase in autoimmune-prone female NZBx NZW F1 mice. International journal of experimental pathology,86 (5), 289-296.

Background Reference 02

Lowenthal, J. W., Tougne, C., MacDonald, H. R., Smith, K. A., Nabholz, M. (1985) . Antigenic stimulation regulates the expression of IL 2 receptors in a cytolytic T lymphocyte clone.The Journal of Immunology,134 (2), 931-939.

Background Reference 03

Huang, B., Zhao, J., Shen, S., Li, H., He, K. L., Shen, G. X., ... Feng, Z. H. (2007) . Listeria monocytogenes promotes tumor growth via tumor cell toll-like receptor 2 signaling.Cancer Research,67 (9), 4346-4352.