CD25 Antibody [PC61.5] (Violet-450)

Product Specifications

Background

The PC61.5 antibody specifically reacts with mouse CD25, the 55 kDa low-affinity Interleukin-2 Receptor alpha chain (IL-2R alpha), expressed on early progenitors of T and B lineage, and on B and T cells. Together with CD122 (IL-2 Receptor beta) and CD 132 (IL-2 Receptor gammac, the common gamma chain), CD25 forms high-affinity receptor complexes for IL-2. Resting B and T cells and natural killer cells do not express IL-2Ralpha. Cd25 is also expressed on the dendritic cells, and it enhances lymphocyte differentiation and activation.The PC61.5 antibody block the binding of IL-2 to both high-affinity and low-affinity receptors.BG Violet 450 conjugate is an alternative to the Pacific Blue, eFluor 450, or BD Horizon V450 dyes. It is excited by the violet (405 nm) laser, with a peak emission of 450nm.

NCBI Gene ID

16184

Swiss Prot

P01590

Host

Rat

Reactivity

Mouse

Clonality

Monoclonal

Clone

PC61.5

Conjugation

Violet-450

Type

Primary Antibodies

Field of Research

Immunology

Purification

The monoclonal antibody was purified utilizing affinity chromatography and unreacted dye was removed from the product.

Concentration

Batch dependent

Buffer

Phosphate-buffered aqueous solution, ≤0.09% Sodium azide, may contain carrier protein/stabilizer, ph7.2.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

The product should be stored undiluted at 4˚ C and should be protected from prolonged exposure to light. Do not freeze.

Fragment

Rat IgG1, lambda

Specificity

Symbol

Il2ra

NCBI Official Name

Interleukin 2 receptor, alpha chain

NCBI Organism

Mus musculus

Background Reference 01

Hayashi, T., Hasegawa, K., Adachi, C. (2005) . Elimination of CD4+ CD25+ T cell accelerates the development of glomerulonephritis during the preactive phase in autoimmune-prone female NZBx NZW F1 mice. International journal of experimental pathology,86 (5), 289-296.

Background Reference 02

Lowenthal, J. W., Tougne, C., MacDonald, H. R., Smith, K. A., Nabholz, M. (1985) . Antigenic stimulation regulates the expression of IL 2 receptors in a cytolytic T lymphocyte clone.The Journal of Immunology,134 (2), 931-939.

Background Reference 03

Huang, B., Zhao, J., Shen, S., Li, H., He, K. L., Shen, G. X., ... Feng, Z. H. (2007) . Listeria monocytogenes promotes tumor growth via tumor cell toll-like receptor 2 signaling.Cancer Research,67 (9), 4346-4352.