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CD45RA Antibody [HI100] (Violet-450)

Product Specifications

Background

The HI100 monoclonal antibody specifically reacts with human CD45RA, the 220 kDa isoform of the human leukocyte common antigen (LCA) found on 40-50% of the peripheral CD4+ T lymphocytes, half of the peripheral CD8+ T lymphocytes and some of the monocytes and B lymphocytes. The CD45RA antigen is expressed by naïve and activated T lymphocytes. The HI100 monoclonal antibody is used as a phenotypic marker to discriminate T lymphocytes subsets.BG Violet 450 conjugate is an alternative to the Pacific Blue, eFluor 450, or BD Horizon V450 dyes. It is excited by the violet (405 nm) laser, with a peak emission of 450nm.

NCBI Gene ID

5788

Swiss Prot

P08575

Host

Mouse

Reactivity

Human

Clonality

Monoclonal

Clone

HI100

Conjugation

Violet-450

Type

Primary Antibodies

Field of Research

Immunology

Purification

The monoclonal antibody was purified utilizing affinity chromatography and unreacted dye was removed from the product.

Concentration

Batch dependent

Buffer

Phosphate-buffered aqueous solution, ≤0.09% Sodium azide, may contain carrier protein/stabilizer, ph7.2.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

The product should be stored undiluted at 4˚ C and should be protected from prolonged exposure to light. Do not freeze.

Product Datasheet

https://www.prosci-inc.com/?datasheet_sku=76-455

Fragment

Mouse IgG2b, kappa

Specificity

The HI100 monoclonal antibody specifically reacts with human CD45RA, the 220 kDa isoform of the human leukocyte common antigen (LCA) found on 40-50% of the peripheral CD4+ T lymphocytes, half of the peripheral CD8+ T lymphocytes and some of the monocytes and B lymphocytes.

Symbol

PTPRC

NCBI Official Name

Protein tyrosine phosphatase, receptor type, C

NCBI Organism

Homo sapiens

Background Reference 01

Knapp W (1989) Leucocyte typing IV: white cell differentiation antigens. Oxford University Press, 1989.

Background Reference 02

Barclay, A. N., Brown, M. H., Law, S. A. K. A., McKnight, A. J., Tomlinson, M. G., van der Merwe, P. A. (1997) .The leucocyte antigen factsbook. Academic Press.

Background Reference 03

Yamada, T., Zhu, D., Saxon, A., Zhang, K. (2002) . CD45 controls interleukin-4-mediated IgE class switch recombination in human B cells through its function as a Janus kinase phosphatase.Journal of Biological Chemistry,277 (32), 28830-28835.

Other Product Names

LCA, LY5, B220, CD45, L-CA, T200, CD45R, GP180, PTPRC

Tested Applications

Flow

Physical Properties

Liquid

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