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CD45 Antibody [HI30] (APC)

Product Specifications

Background

The HI30 monoclonal antibody specifically reacts with the 180 kDa, 190 kDa, 205 kDa, and 220 kDa isoforms of the human leukocyte common antigen (LCA) CD45. It is expressed on lymphocytes, granulocytes, monocytes, thymocytes, and eosinophils, but not on mature erythrocytes, platelets, mature erythroid cells of bone marrow, and non-hematopoietic tissues. CD45 is essential for T cell activation and the tyrosine phosphatase activity of its intracellular region is integral for signal transduction.

NCBI Gene ID

5788

Swiss Prot

P08575

Host

Mouse

Reactivity

Human

Clonality

Monoclonal

Clone

HI30

Conjugation

APC

Type

Primary Antibodies

Field of Research

Immunology

Purification

The monoclonal antibody was purified utilizing affinity chromatography and unreacted dye was removed from the product.

Concentration

Batch dependent

Buffer

Phosphate-buffered aqueous solution, ≤0.09% Sodium azide, may contain carrier protein/stabilizer, ph7.2.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

The product should be stored undiluted at 4˚ C and should be protected from prolonged exposure to light. Do not freeze.

Product Datasheet

https://www.prosci-inc.com/?datasheet_sku=76-451

Fragment

Mouse IgG1, kappa

Specificity

The HI30 monoclonal antibody specifically reacts with the 180 kDa, 190 kDa, 205 kDa, and 220 kDa isoforms of the human leukocyte common antigen (LCA) CD45.

Symbol

PTPRC

NCBI Official Name

Protein tyrosine phosphatase, receptor type, C

NCBI Organism

Homo sapiens

Background Reference 01

Knapp W (1989) Leucocyte typing IV: white cell differentiation antigens. Oxford University Press, 1989.

Background Reference 02

Ninomiya, M., Abe, A., Katsumi, A., Xu, J., Ito, M., Arai, F., ... Naoe, T. (2006) . Homing, proliferation and survival sites of human leukemia cells in vivo in immunodeficient mice.Leukemia,21 (1), 136-142.

Background Reference 03

Yoshino, N., AMI, Y., TERAO, K., TASHIRO, F., HONDA, M. (2000) . Upgrading of flow cytometric analysis for absolute counts, cytokines and other antigenic molecules of cynomolgus monkeys (Macaca fascicularis) by using anti-human cross-reactive antibodies.Experimental Animals,49 (2), 97-110.

Other Product Names

LCA, LY5, B220, CD45, L-CA, T200, CD45R, GP180, PTPRC

Tested Applications

Flow

Physical Properties

Liquid

Curated Selection

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