CD64 Antibody [10.1]

Product Specifications

Background

The 10.1 monoclonal antibody specifically reacts with human CD64, a 75kDA type I transmembrane glycoprotein that is the high affinity receptor for IgG. CD64 is expressed on macrophages and dendritic cells and is involved in antibody-dependent cellular toxicity, cytokine release phagocytosis, and endocytosis. The 10.1 antibody is reported to recognize the EC3 epitope of CD64.

NCBI Gene ID

2209

Swiss Prot

P12314

Host

Mouse

Reactivity

Human

Clonality

Monoclonal

Clone

10.1

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Innate Immunity

Purification

The monoclonal antibody was purified utilizing affinity chromatography. The endotoxin level is determined by LAL test to be less than 0.01 EU/µg of the protein.

Concentration

Batch dependent

Buffer

Phosphate-buffered aqueous solution, ph7.2.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

The product should be stored undiluted at 4˚ C . Do not freeze.

Fragment

Mouse IgG1, kappa

Specificity

The 15.2 antibody reacts with the 85-110 kDa intracellular adhesion molecule-1 (ICAM-1), a member of the Ig superfamily which acts as a ligand for the Lymphocyte Function-Associated Antigen-1 (LFA-1) .

Symbol

FCGR1A

NCBI Official Name

Fc fragment of IgG, high affinity Ia, receptor

NCBI Organism

Homo sapiens

Background Reference 01

Schlossman, S. F. (1995) .Leucocyte typing V: White cell differentiation antigens: Proceedings of the Fifth International Workshop and Conference, Held in Boston, USA 3-7 November, 1993. Oxford University Press.

Background Reference 02

Dougherty, G. J., Selvendran, Y., Murdoch, S., Palmer, D. G., Hogg, N. (1987) . The human mononuclear phagocyte high‐affinity Fc receptor, FcRI, defined by a monoclonal antibody, 10.1.European journal of immunology,17 (10), 1453-1459.

Background Reference 03

Indik, Z. K., Hunter, S., Huang, M. M., Pan, X. Q., Chien, P., Kelly, C., ... Schreiber, A. D. (1994) . The high affinity Fc gamma receptor (CD64) induces phagocytosis in the absence of its cytoplasmic domain: the gamma subunit of Fc gamma RIIIA imparts phagocytic function to Fc gamma RI.Experimental hematology,22 (7), 599-606.