CD54 Antibody [15.2]

Product Specifications

Background

The 15.2 antibody reacts with the 85-110 kDa intracellular adhesion molecule-1 (ICAM-1), a member of the Ig superfamily which acts as a ligand for the Lymphocyte Function-Associated Antigen-1 (LFA-1) . ICAM-1 is also known CD 54 and is expressed on non-hematopoietic cells of vascular endothelial, thymic epithelial, fibroblasts lineages, and on hematopoietic cells like macrophages, mitogen-stimulated T-lymphoblasts, dendritic cells in tonsils, lymph nodes and Peyer’s patches, and germinal center B cells.Inflammatory mediators (IL-1, TNF, IFN-gamma) enhance the production of ICAM-1 on fibroblasts and endothelial cells within few hours. Thus, ICAM-1 seems to be the marker of inflammatory reactions.

NCBI Gene ID

3383

Swiss Prot

P05362

Host

Mouse

Reactivity

Human

Clonality

Monoclonal

Clone

15.2

Conjugation

Unconjugated

Type

Primary Antibodies, Functional Antibodies

Field of Research

Immunology

Purification

The monoclonal antibody was purified utilizing affinity chromatography and unreacted dye was removed from the product.

Concentration

Batch dependent

Buffer

Phosphate-buffered aqueous solution, ≤0.09% Sodium azide, may contain carrier protein/stabilizer, ph7.2.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

The product should be stored undiluted at 4˚ C and should be protected from prolonged exposure to light. Do not freeze.

Fragment

Mouse IgG1

Specificity

Symbol

ICAM1

NCBI Official Name

Intercellular adhesion molecule 1

NCBI Organism

Homo sapiens

Background Reference 01

Bhattacharya, A. L. O. K., Dorf, M. E., Springer, T. A. (1981) . A shared alloantigenic determinant on Ia antigens encoded by the IA and IE subregions: evidence for I region gene duplication. The Journal of Immunology,127 (6), 2488-2495.

Background Reference 02

Mendiratta, S. K., Singh, N., Bal, V., Rath, S. (1996) . Analysis of T-cell hybridomas with an unusual MHC class II-dependent ligand specificity.Immunology,89 (2), 238-244.

Background Reference 03

Unternaehrer, J. J., Chow, A., Pypaert, M., Inaba, K., Mellman, I. (2007) . The tetraspanin CD9 mediates lateral association of MHC class II molecules on the dendritic cell surface.Proceedings of the National Academy of Sciences,104 (1), 234-239.