CD14 Antibody [Sa2-8] (FITC)

Product Specifications

Background

The Sa2-8 monoclonal antibody specifically reacts with mouse CD14, a cell surface anchored glycoprotein that is primarily expressed on macrophages and is secreted in certain conditions. CD14 associates with LPS and LBP to form a receptor complex, which signals specifically in response to bacterial lipopolysaccharide (LPS) binding. The Sa2-8 antibody exhibits a weak antagonistic activate in relation to TNF alpha production and NF-kappa B activation.

NCBI Gene ID

929

Swiss Prot

P08571

Host

Rat

Reactivity

Mouse

Clonality

Monoclonal

Clone

Sa2-8

Conjugation

FITC

Type

Primary Antibodies

Field of Research

Innate Immunity

Purification

The monoclonal antibody was purified utilizing affinity chromatography and unreacted dye was removed from the product.

Concentration

Batch dependent

Buffer

Phosphate-buffered aqueous solution, ≤0.09% Sodium azide, may contain carrier protein/stabilizer, ph7.2.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

The product should be stored undiluted at 4˚ C and should be protected from prolonged exposure to light. Do not freeze.

Fragment

Rat IgG2a, kappa

Specificity

The Sa2-8 monoclonal antibody specifically reacts with mouse CD14, a cell surface anchored glycoprotein that is primarily expressed on macrophages and is secreted in certain conditions.

Symbol

CD14

NCBI Official Name

CD14 molecule

NCBI Organism

Homo sapiens

Background Reference 01

Akashi, S., Saitoh, S. I., Wakabayashi, Y., Kikuchi, T., Takamura, N., Nagai, Y., ... Miyake, K. (2003) . Lipopolysaccharide Interaction with Cell Surface Toll-like Receptor 4-MD-2 Higher Affinity than That with MD-2 or CD14.The Journal of experimental medicine,198 (7), 1035-1042.

Background Reference 02

Oliva, C., Turnbough, C. L., Kearney, J. F. (2009) . CD14-Mac-1 interactions in Bacillus anthracis spore internalization by macrophages.Proceedings of the National Academy of Sciences,106 (33), 13957-13962.