CD4 Antibody [GK1.5] (FITC)

Product Specifications

Background

The GK1.5 monoclonal antibody specifically binds with the mouse CD4 molecule, also known as L3T4, a 55 kDa differentiation antigen which binds to the MHC class II. CD4 is expressed on most thymocytes, a subpopulation of mature T lymphocytes, dendritic cells, pluripotent hematopoietic stem cells, B cell precursors, and lymphoid precursors inside the thymus. It is also expressed on the mouse egg cell membrane, enhancing adhesion to MHC class II bearing sperm. By interaction with MHC class II on the surface of APC, CD4 initiates the development of T lymphocytes and helps the optimum functioning of mature T lymphocytes. The binding of the GK1.5 antibody blocks the binding of the Anti-Mouse CD4 RM4-5 antibody.

NCBI Gene ID

12504

Swiss Prot

P06332

Host

Rat

Reactivity

Mouse

Clonality

Monoclonal

Clone

GK1.5

Conjugation

FITC

Type

Primary Antibodies

Field of Research

Immunology

Purification

The monoclonal antibody was purified utilizing affinity chromatography and unreacted dye was removed from the product.

Concentration

Batch dependent

Buffer

Phosphate-buffered aqueous solution, ≤0.09% Sodium azide, may contain carrier protein/stabilizer, ph7.2.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

The product should be stored undiluted at 4˚ C and should be protected from prolonged exposure to light. Do not freeze.

Fragment

Rat IgG2b, kappa

Specificity

The GK1.5 monoclonal antibody specifically binds with the mouse CD4 molecule, also known as L3T4, a 55 kDa differentiation antigen which binds to the MHC class II.

Symbol

Cd4

NCBI Official Name

CD4 antigen

NCBI Organism

Mus musculus

Background Reference 01

Dialynas, D. P., Quan, Z. S., Wall, K. A., Pierres, A., Quintans, J., Loken, M. R., ... Fitch, F. W. (1983) . Characterization of the murine T cell surface molecule, designated L3T4, identified by monoclonal antibody GK1. 5: similarity of L3T4 to the human Leu-3/T4 molecule.The Journal of Immunology,131 (5), 2445-2451.

Background Reference 02

Bosselut, R., Zhang, W., Ashe, J. M., Kopacz, J. L., Samelson, L. E., Singer, A. (1999) . Association of the adaptor molecule LAT with CD4 and CD8 coreceptors identifies a new coreceptor function in T cell receptor signal transduction.The Journal of experimental medicine,190 (10), 1517-1526.

Background Reference 03

Ghobrial, R. R., Boublik, M., Winn, H. J., Auchincloss Jr, H. (1989) . In Vivo use of monoclonal antibodies against murine T cell antigens.Clinical immunology and immunopathology,52 (3), 486-506.