CD3e Antibody [145-2C11]
Product Specifications
Background
The 145-2C11 monoclonal antibody reacts with mouse CD3e, the 20 kDa epsilon chain of the TCR complex. Together with the gamma and delta subunits of CD3, the epsilon subunits are involved in the assembly, trafficking, and surface expression of T-cell receptor complex. CD3 is expressed on thymocytes, mature T cells, and natural killer T cells, and the epsilon chain enhances the antigen recognition.The 145-2C11 antibody binds to the TCR complex and, depending on the conditions, initiates T cells activation, proliferation, and apoptosis. The soluble antibody seems to block lysis of target cells by antigen-specific cytotoxic T lymphocytes. The 145-2C11 antibody does not cross-react with the rat leukocytes, and it is used as a phenotypic marker for mouse T lymphocytes.
NCBI Gene ID
12501
Swiss Prot
P22646
Host
Hamster
Reactivity
Mouse
Clonality
Monoclonal
Clone
145-2C11
Conjugation
Unconjugated
Type
Primary Antibodies, Functional Antibodies
Field of Research
Immunology
Purification
The monoclonal antibody was purified utilizing affinity chromatography. The endotoxin level is determined by LAL test to be less than 0.01 EU/µg of the protein.
Concentration
Batch dependent
Buffer
Phosphate-buffered aqueous solution, ph7.2.
Modification
None
Shipping Conditions
Blue Ice
Storage Conditions
The product should be stored undiluted at 4˚ C . Do not freeze.
Product Datasheet
https://www.prosci-inc.com/?datasheet_sku=76-260
Fragment
Armenian Hamster IgG
Specificity
The 145-2C11 monoclonal antibody reacts with mouse CD3e, the 20 kDa epsilon chain of the TCR complex.
Symbol
Cd3e
NCBI Official Name
CD3 antigen, epsilon polypeptide
NCBI Organism
Mus musculus
Background Reference 01
Leo, O., Foo, M., Sachs, D. H., Samelson, L. E., Bluestone, J. A. (1987) . Identification of a monoclonal antibody specific for a murine T3 polypeptide.Proceedings of the National Academy of Sciences,84 (5), 1374-1378.
Background Reference 02
Nakano, H., Yamazaki, T., Miyatake, S., Nozaki, N., Kikuchi, A., Saito, T. (1996) . Specific Interaction of Topoisomerase II and the CD3 Chain of the T Cell Receptor Complex.Journal of Biological Chemistry,271 (11), 6483-6489.
Background Reference 03
Salmond, R. J., Filby, A., Pirinen, N., Magee, A. I., Zamoyska, R. (2011) . Mislocalization of Lck impairs thymocyte differentiation and can promote development of thymomas.Blood,117 (1), 108-117.
Other Product Names
CD3, T3e, AI504783, CD3epsilon, Cd3e
Tested Applications
Flow, Functional Assays, IHC, IF, IP, WB
Physical Properties
Liquid
Curated Selection
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