CD3e Antibody [145-2C11]

Product Specifications

Background

The 145-2C11 monoclonal antibody reacts with mouse CD3e, the 20 kDa epsilon chain of the TCR complex. Together with the gamma and delta subunits of CD3, the epsilon subunits are involved in the assembly, trafficking, and surface expression of T-cell receptor complex. CD3 is expressed on thymocytes, mature T cells, and natural killer T cells, and the epsilon chain enhances the antigen recognition.The 145-2C11 antibody binds to the TCR complex and, depending on the conditions, initiates T cells activation, proliferation, and apoptosis. The soluble antibody seems to block lysis of target cells by antigen-specific cytotoxic T lymphocytes. The 145-2C11 antibody does not cross-react with the rat leukocytes, and it is used as a phenotypic marker for mouse T lymphocytes.

NCBI Gene ID

12501

Swiss Prot

P22646

Host

Hamster

Reactivity

Mouse

Clonality

Monoclonal

Clone

145-2C11

Conjugation

Unconjugated

Type

Primary Antibodies, Functional Antibodies

Field of Research

Immunology

Purification

The monoclonal antibody was purified utilizing affinity chromatography. The endotoxin level is determined by LAL test to be less than 0.01 EU/µg of the protein.

Concentration

Batch dependent

Buffer

Phosphate-buffered aqueous solution, ph7.2.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

The product should be stored undiluted at 4˚ C . Do not freeze.

Fragment

Armenian Hamster IgG

Specificity

The 145-2C11 monoclonal antibody reacts with mouse CD3e, the 20 kDa epsilon chain of the TCR complex.

Symbol

Cd3e

NCBI Official Name

CD3 antigen, epsilon polypeptide

NCBI Organism

Mus musculus

Background Reference 01

Leo, O., Foo, M., Sachs, D. H., Samelson, L. E., Bluestone, J. A. (1987) . Identification of a monoclonal antibody specific for a murine T3 polypeptide.Proceedings of the National Academy of Sciences,84 (5), 1374-1378.

Background Reference 02

Nakano, H., Yamazaki, T., Miyatake, S., Nozaki, N., Kikuchi, A., Saito, T. (1996) . Specific Interaction of Topoisomerase II and the CD3 Chain of the T Cell Receptor Complex.Journal of Biological Chemistry,271 (11), 6483-6489.

Background Reference 03

Salmond, R. J., Filby, A., Pirinen, N., Magee, A. I., Zamoyska, R. (2011) . Mislocalization of Lck impairs thymocyte differentiation and can promote development of thymomas.Blood,117 (1), 108-117.