CD3 Antibody [17A2]

Product Specifications

Background

The 17A2 monoclonal antibody specifically reacts with the mouse T lymphocytes receptor (TCR) associated CD3 complex, resulting in cellular activation and proliferation. CD3 is expressed by thymocytes and mature lymphocytes, and contains gamma, delta, and epsilon subunits, involved in the assembly, trafficking, and surface expression of T-cell receptor complex. The interaction between the T lymphocytes and the 17A2 antibody can be blocked by the 145-2C11 anti-CD3e antibody, demonstrating that the 17A2 antibody recognizes the CD3 epsilon chain.

NCBI Gene ID

12501

Swiss Prot

P22646

Host

Rat

Reactivity

Mouse

Clonality

Monoclonal

Clone

17A2

Conjugation

Unconjugated

Type

Primary Antibodies, Functional Antibodies

Field of Research

Immunology

Purification

The monoclonal antibody was purified utilizing affinity chromatography. The endotoxin level is determined by LAL test to be less than 0.01 EU/µg of the protein.

Concentration

Batch dependent

Buffer

Phosphate-buffered aqueous solution, ph7.2.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

The product should be stored undiluted at 4˚ C . Do not freeze.

Fragment

Rat IgG2b, kappa

Specificity

The 17A2 monoclonal antibody specifically reacts with the mouse T lymphocytes receptor (TCR) associated CD3 complex, resulting in cellular activation and proliferation.

Symbol

Cd3e

NCBI Official Name

CD3 antigen, epsilon polypeptide

NCBI Organism

Mus musculus

Background Reference 01

Miescher, G. C., Schreyer, M., MacDonald, H. R. (1989) . Production and characterization of a rat monoclonal antibody against the murine CD3 molecular complex.Immunology letters,23 (2), 113-118.

Background Reference 02

Mysliwietz, J., Thierfelder, S. (1992) . Antilymphocytic antibodies and marrow transplantation. XII. Suppression of graft-versus-host disease by T-cell-modulating and depleting antimouse CD3 antibody is most effective when preinjected in the marrow recipient.Blood,80 (10), 2661-2667.

Background Reference 03

Wu, L., Antica, M., Johnson, G. R., Scollay, R., Shortman, K. (1991) . Developmental potential of the earliest precursor cells from the adult mouse thymus.The Journal of experimental medicine,174 (6), 1617-1627.