CD3 Antibody [Hit3a]

Product Specifications

Background

The Hit3a monoclonal antibody specifically reacts with the epsilon chain of the CD3/T lymphocyte antigen receptor complex. The CD3 complex is part of the TCR complex, expressed by all mature T lymphocytes and by the thymocyte lineage. CD3 enhances the antigen recognition by signal transduction.The HIT3a antibody cross-links with the TCR complex, initiating the cellular activation and proliferation, but it cannot be used for intracellular CD3 staining, as it is able to stain only the surface CD3 complex.

NCBI Gene ID

916

Swiss Prot

P07766

Host

Mouse

Reactivity

Human

Clonality

Monoclonal

Clone

Hit3a

Conjugation

Unconjugated

Type

Primary Antibodies, Functional Antibodies

Field of Research

Immunology

Purification

The monoclonal antibody was purified utilizing affinity chromatography.

Concentration

Batch dependent

Buffer

Phosphate-buffered aqueous solution, ≤0.09% Sodium azide, may contain carrier protein/stabilizer, ph7.2.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

The product should be stored undiluted at 4˚ C . Do not freeze.

Fragment

Mouse IgG2a, kappa

Specificity

The Hit3a monoclonal antibody specifically reacts with the epsilon chain of the CD3/T lymphocyte antigen receptor complex.

Symbol

CD3E

NCBI Official Name

CD3e molecule, epsilon (CD3-TCR complex)

NCBI Organism

Homo sapiens

Background Reference 01

McMichael, A. J. (1987) . Leucocyte typing III.Oxford University Press, Oxford. Norton AJ, Isaacson PG (1985)

Background Reference 02

Knapp W (1989) Leucocyte typing IV: white cell differentiation antigens. Oxford University Press, 1989.

Background Reference 03

Schlossman, S., L. Bloumsell, et al. eds (1995) . Leucocyte Typing V: White Cell Differentiation Antigens. Oxford University Press. New York

Background Reference 04

Lanier, L. L., Allison, J. P., Phillips, J. H. (1986) . Correlation of cell surface antigen expression on human thymocytes by multi-color flow cytometric analysis: implications for differentiation.The Journal of Immunology,137 (8), 2501-2507.