CD62L Antibody [MEL-14] (APC)

Product Specifications

Background

The MEL-14 monoclonal antibody specifically reacts with L- selectin (CD62L), a receptor with lectin-like and Epidermal Growth Factor-like domains. The weight of the CD62L molecules depend on their origin: 74 kDa (on lymphocytes) or 95 kDa (on neutrophils) .In the Mouseorganism, CD62L can be expressed by most thymocytes, on subsets of B and T lymphocytes, monocytes, eosinophils, and neutrophils. The L-selectin binds sulfated, fucosylated, and glycosylated glycoproteins (MadCAM-1, GLYCAM-1, and CD 34) . It mediates the migration of lymphocytes to the site of inflammation and their return to the peripheral lymphoid tissues and to the HEV (high endothelial venules) . In vitro, L-selectin inhibits this binding and the lymphocyte extravasation into peripheral lymph nodes.

NCBI Gene ID

20343

Swiss Prot

P18337

Host

Rat

Reactivity

Mouse

Clonality

Monoclonal

Clone

MEL-14

Conjugation

APC

Type

Primary Antibodies

Field of Research

Immunology

Purification

The monoclonal antibody was purified utilizing affinity chromatography and unreacted dye was removed from the product.

Concentration

Batch dependent

Buffer

Phosphate-buffered aqueous solution, ≤0.09% Sodium azide, may contain carrier protein/stabilizer, ph7.2.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

The product should be stored undiluted at 4˚ C and should be protected from prolonged exposure to light. Do not freeze.

Fragment

Rat IgG2a, kappa

Specificity

The MEL-14 monoclonal antibody specifically reacts with L- selectin (CD62L), a receptor with lectin-like and Epidermal Growth Factor-like domains.

Symbol

Sell

NCBI Official Name

Selectin, lymphocyte

NCBI Organism

Mus musculus

Background Reference 01

Gallatin, W. M., Weissman, I. L., Butcher, E. C. (1982) . A cell-surface molecule involved in organ-specific homing of lymphocytes.Nature,304 (5921), 30-34.

Background Reference 02

Siegelman, M. H., Cheng, I. C., Weissman, I. L., Wakeland, E. K. (1990) . The mouse lymph node homing receptor is identical with the lymphocyte cell surface marker Ly-22: role of the EGF domain in endothelial binding.Cell,61 (4), 611-622.

Background Reference 03

Pizcueta, P., Luscinskas, F. W. (1994) . Monoclonal antibody blockade of L-selectin inhibits mononuclear leukocyte recruitment to inflammatory sites in vivo.The American journal of pathology,145 (2), 461.