CD62L Antibody [MEL-14] (PE-Cy7)

Product Specifications

Background

The MEL-14 monoclonal antibody specifically reacts with L- selectin (CD62L), a receptor with lectin-like and Epidermal Growth Factor-like domains. The weight of the CD62L molecules depend on their origin: 74 kDa (on lymphocytes) or 95 kDa (on neutrophils) .In the Mouseorganism, CD62L can be expressed by most thymocytes, on subsets of B and T lymphocytes, monocytes, eosinophils, and neutrophils. The L-selectin binds sulfated, fucosylated, and glycosylated glycoproteins (MadCAM-1, GLYCAM-1, and CD 34) . It mediates the migration of lymphocytes to the site of inflammation and their return to the peripheral lymphoid tissues and to the HEV (high endothelial venules) . In vitro, L-selectin inhibits this binding and the lymphocyte extravasation into peripheral lymph nodes.

NCBI Gene ID

20343

Swiss Prot

P18337

Host

Rat

Reactivity

Mouse

Clonality

Monoclonal

Clone

MEL-14

Conjugation

PE-Cy7

Type

Primary Antibodies

Field of Research

Immunology

Purification

The monoclonal antibody was purified utilizing affinity chromatography and unreacted dye was removed from the product.

Concentration

Batch dependent

Buffer

Phosphate-buffered aqueous solution, ≤0.09% Sodium azide, may contain carrier protein/stabilizer, ph7.2.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

The product should be stored undiluted at 4˚ C and should be protected from prolonged exposure to light. Do not freeze.

Fragment

Rat IgG2a, kappa

Specificity

The MEL-14 monoclonal antibody specifically reacts with L- selectin (CD62L), a receptor with lectin-like and Epidermal Growth Factor-like domains.

Symbol

Sell

NCBI Official Name

Selectin, lymphocyte

NCBI Organism

Mus musculus

Background Reference 01

Gallatin, W. M., Weissman, I. L., Butcher, E. C. (1982) . A cell-surface molecule involved in organ-specific homing of lymphocytes.Nature,304 (5921), 30-34.

Background Reference 02

Siegelman, M. H., Cheng, I. C., Weissman, I. L., Wakeland, E. K. (1990) . The mouse lymph node homing receptor is identical with the lymphocyte cell surface marker Ly-22: role of the EGF domain in endothelial binding.Cell,61 (4), 611-622.

Background Reference 03

Pizcueta, P., Luscinskas, F. W. (1994) . Monoclonal antibody blockade of L-selectin inhibits mononuclear leukocyte recruitment to inflammatory sites in vivo.The American journal of pathology,145 (2), 461.