CD32 Antibody [6C4]

Product Specifications

Background

The 6C4 monoclonal antibody specifically recognizes two isoforms of human CD32, Fc gamma RIIA and Fc gamma RIIB. From alternative mRNA splicing, 6 isoforms of CD32 have been reported. CD32 is a 40 kDA polymorphic transmembrane glycoprotein expressed on monocytes, macrophages, graulocytes, B cells, and platelets. The receptor has low affinity toward IgG and is involved in immunomodulation, phagocytosis, and the inflammation response. The 6C4 antibody in a rosette blocking assay is reported to inhibit Ig binding

NCBI Gene ID

2212

Swiss Prot

P12318

Host

Mouse

Reactivity

Human

Clonality

Monoclonal

Clone

6C4

Conjugation

Unconjugated

Type

Primary Antibodies, Functional Antibodies

Field of Research

Innate Immunity

Purification

The monoclonal antibody was purified utilizing affinity chromatography. The endotoxin level is determined by LAL test to be less than 0.01 EU/µg of the protein.

Concentration

Batch dependent

Buffer

Phosphate-buffered aqueous solution, ph7.2.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

The product should be stored undiluted at 4˚ C . Do not freeze.

Fragment

Mouse IgG1, kappa

Specificity

The 6C4 monoclonal antibody specifically recognizes two isoforms of human CD32, Fc gamma RIIA and Fc gamma RIIB.

Symbol

FCGR2A

NCBI Official Name

Fc fragment of IgG, low affinity IIa, receptor (CD32)

NCBI Organism

Homo sapiens

Background Reference 01

VELY, F., GRUEL, N., MONCUIT, J., COCHET, O., ROUARD, H., DARE, S., ... TEILLAUD, J. L. (1997) . A new set of monoclonal antibodies against human Fc-gamma RII (CD32) and Fc-gamma RIII (CD16) : characterization and use in various assays.Hybridoma,16 (6), 519-528.

Background Reference 02

Liebert, M. A. (1997) . 6C4, 2B2, 3D3.Hybridoma,16 (6) .

Background Reference 03

Bouhlal, H., Martinvalet, D., Teillaud, J. L., Fridman, C., Kazatchkine, M. D., Bayry, J., ... Kaveri, S. V. (2014) . Natural Autoantibodies to Fc-gamma Receptors in Intravenous Immunoglobulins.Journal of clinical immunology, 1-8.