CD11b Antibody [M1/70]

Product Specifications

Background

The M1/70 monoclonal antibody specifically reacts with the 170 kDa alphaM integrin chain of mouse CD11b from the Mac-1 integrin (CD11b/CD18) . Mac-1 binds to C3bi, CD54 (ICAM-1), and fibrinogen, and it is expressed by granulocytes, macrophages, NK cells, myeloid-derived dendritic cells, microglia, activated lymphocytes, and mouse B-1 cells. The expression is up-regulated on activated neutrophils at the same time that L-selectin is shed from the cell surface. The M1/70 antibody is used for the detection of monocytes, granulocytes, and a subset of natural killer cells in human peripheral blood.M1/70 blocks C3bi binding and cell adherence, but not cell-mediated lysis and it cross-reacts with human CD11b.

NCBI Gene ID

3684

Swiss Prot

P11215

Host

Rat

Reactivity

Human, Mouse

Clonality

Monoclonal

Clone

M1/70

Conjugation

Unconjugated

Type

Primary Antibodies, Functional Antibodies

Field of Research

Other

Purification

The monoclonal antibody was purified utilizing affinity chromatography.

Concentration

Batch dependent

Buffer

Phosphate-buffered aqueous solution, ph7.2.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

The product should be stored undiluted at 4˚ C . Do not freeze.

Fragment

Rat IgG2b, kappa

Specificity

The M1/70 monoclonal antibody specifically reacts with the 170 kDa alphaM integrin chain of mouse CD11b from the Mac-1 integrin (CD11b/CD18) .

Symbol

ITGAM

NCBI Official Name

Integrin, alpha M (complement component 3 receptor 3 subunit)

NCBI Organism

Homo sapiens

Background Reference 01

Sanchez-Madrid, F., Simon, P., Thompson, S., Springer, T. A. (1983) . Mapping of antigenic and functional epitopes on the alpha-and beta-subunits of two related mouse glycoproteins involved in cell interactions, LFA-1 and Mac-1.The Journal of experimental medicine,158 (2), 586-602.

Background Reference 02

Ault, K. A., Springer, T. A. (1981) . Cross-reaction of a rat-anti-mouse phagocyte-specific monoclonal antibody (anti-Mac-1) with human monocytes and natural killer cells.The Journal of Immunology,126 (1), 359-364.

Background Reference 03

Springer, T., Galfre, G., Secher, D. S., Milstein, C. (1978) . Monoclonal xenogeneic antibodies to murine cell surface antigens: identification of novel leukocyte differentiation antigens.European journal of immunology,8 (8), 539-551.