CD11b (activation epitope) Antibody [CBRM1/5]

Product Specifications

Background

The CBRM1/5 monoclonal antibody binds with the 165 kDa human adhesion glycoprotein CD11b, which forms, together with the 95 kDa CD18 (integrin β2) a complex known as Mac-1. CD11b is expressed on the surface of activated lymphocytes, a subset of natural killer cells, granulocytes, and monocytes. It functions as a receptor in cell-cell and cell-matrix interactions and as a receptor for iC3b, ICAM-1, ICAM-2, and ICAM-3 intercellular adhesion molecules. The CBRM1/5 specifically reacts with the activation-specific epitope of the Mac-1 molecule, in the I domain on the alpha chain near the ligand binding site. The antibody has been reported to inhibit the cellular adhesion mediated by Mac-1.

NCBI Gene ID

3684

Swiss Prot

P11215

Host

Mouse

Reactivity

Human

Clonality

Monoclonal

Clone

CBRM1/5

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Innate Immunity

Purification

The monoclonal antibody was purified utilizing affinity chromatography. The endotoxin level is determined by LAL test to be less than 0.01 EU/µg of the protein.

Concentration

Batch dependent

Buffer

Phosphate-buffered aqueous solution, ph7.2.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

The product should be stored undiluted at 4˚ C . Do not freeze.

Fragment

Mouse IgG1, kappa

Specificity

The CBRM1/5 monoclonal antibody binds with the 165 kDa human adhesion glycoprotein CD11b, which forms, together with the 95 kDa CD18 (integrin β2) a complex known as Mac-1.

Symbol

ITGAM

NCBI Official Name

Integrin, alpha M (complement component 3 receptor 3 subunit)

NCBI Organism

Homo sapiens

Background Reference 01

Oxvig, C., Lu, C., Springer, T. A. (1999) . Conformational changes in tertiary structure near the ligand binding site of an integrin I domain.Proceedings of the National Academy of Sciences,96 (5), 2215-2220.

Background Reference 02

Diamond, M. S., Springer, T. A. (1993) . A subpopulation of Mac-1 (CD11b/CD18) molecules mediates neutrophil adhesion to ICAM-1 and fibrinogen.The Journal of cell biology,120 (2), 545-556.

Background Reference 03

Shimaoka, M., Shifman, J. M., Jing, H., Takagi, J., Mayo, S. L., Springer, T. A. (2000) . Computational design of an integrin I domain stabilized in the open high affinity conformation.Nature Structural Molecular Biology,7 (8), 674-678.