F4/80 Antigen Antibody [BM8.1] (PerCP-Cy5.5)

Product Specifications

Background

The BM8.1 monoclonal antibody specifically binds to the Mouse125 kDa F4/80 antigen, expressed by most mature macrophages. F4/80 is a transmembrane protein used as a marker of macrophages, although it is also expressed on Kupffer and Langerhans cells. The expression of F4/80 antigen is upregulated on bone marrow cells stimulated in vitro with the macrophage colony stimulating factor. The F4/80 antigen is a requirement for the induction of CD8 T cells-mediated peripheral tolerance.

NCBI Gene ID

13733

Swiss Prot

Q61549

Host

Rat

Reactivity

Mouse

Clonality

Monoclonal

Clone

BM8.1

Conjugation

PerCP-Cy5.5

Type

Primary Antibodies

Field of Research

Innate Immunity

Purification

The monoclonal antibody was purified utilizing affinity chromatography and unreacted dye was removed from the product.

Concentration

Batch dependent

Buffer

Phosphate-buffered aqueous solution, ≤0.09% Sodium azide, may contain carrier protein/stabilizer, ph7.2.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

The product should be stored undiluted at 4˚ C and should be protected from prolonged exposure to light. Do not freeze.

Fragment

Rat IgG2a, kappa

Specificity

The BM8.1 monoclonal antibody specifically binds to the Mouse125 kDa F4/80 antigen, expressed by most mature macrophages.

Symbol

Emr1

NCBI Official Name

EGF-like module containing, mucin-like, hormone receptor-like sequence 1

NCBI Organism

Mus musculus

Background Reference 01

Zwadlo, G., Brcker, E. B., Von Bassewitz, D. B., Feige, U., Sorg, C. (1985) . A monoclonal antibody to a differentiation antigen present on mature human macrophages and absent from monocytes.The Journal of Immunology,134 (3), 1487-1492.

Background Reference 02

Leenen, P. J., de Bruijn, M. F., Voerman, J. S., Campbell, P. A., van Ewijk, W. (1994) . Markers of mouse macrophage development detected by monoclonal antibodies.Journal of immunological methods,174 (1), 5-19.

Background Reference 03

Schaller, E., Macfarlane, A. J., Rupec, R. A., Gordon, S., McKnight, A. J., Pfeffer, K. (2002) . Inactivation of the F4/80 glycoprotein in the mouse germ line.Molecular and cellular biology,22 (22), 8035-8043.