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F4/80 Antigen Antibody [BM8.1] (Violet-450)

Product Specifications

Background

The BM8.1 monoclonal antibody specifically binds to the Mouse125 kDa F4/80 antigen, expressed by most mature macrophages. F4/80 is a transmembrane protein used as a marker of macrophages, although it is also expressed on Kupffer and Langerhans cells. The expression of F4/80 antigen is upregulated on bone marrow cells stimulated in vitro with the macrophage colony stimulating factor. The F4/80 antigen is a requirement for the induction of CD8 T cells-mediated peripheral tolerance.BG Violet 450 conjugate is an alternative to the Pacific Blue, eFluor 450, or BD Horizon V450 dyes. It is excited by the violet (405 nm) laser, with a peak emission of 450nm.

NCBI Gene ID

13733

Swiss Prot

Q61549

Host

Rat

Reactivity

Mouse

Clonality

Monoclonal

Clone

BM8.1

Conjugation

Violet-450

Type

Primary Antibodies

Field of Research

Innate Immunity

Purification

The monoclonal antibody was purified utilizing affinity chromatography and unreacted dye was removed from the product.

Concentration

Batch dependent

Buffer

Phosphate-buffered aqueous solution, ≤0.09% Sodium azide, may contain carrier protein/stabilizer, ph7.2.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

The product should be stored undiluted at 4˚ C and should be protected from prolonged exposure to light. Do not freeze.

Product Datasheet

https://www.prosci-inc.com/?datasheet_sku=76-055

Fragment

Rat IgG2a, kappa

Specificity

The BM8.1 monoclonal antibody specifically binds to the Mouse125 kDa F4/80 antigen, expressed by most mature macrophages.

Symbol

Emr1

NCBI Official Name

EGF-like module containing, mucin-like, hormone receptor-like sequence 1

NCBI Organism

Mus musculus

Background Reference 01

Zwadlo, G., Brcker, E. B., Von Bassewitz, D. B., Feige, U., Sorg, C. (1985) . A monoclonal antibody to a differentiation antigen present on mature human macrophages and absent from monocytes.The Journal of Immunology,134 (3), 1487-1492.

Background Reference 02

Leenen, P. J., de Bruijn, M. F., Voerman, J. S., Campbell, P. A., van Ewijk, W. (1994) . Markers of mouse macrophage development detected by monoclonal antibodies.Journal of immunological methods,174 (1), 5-19.

Background Reference 03

Schaller, E., Macfarlane, A. J., Rupec, R. A., Gordon, S., McKnight, A. J., Pfeffer, K. (2002) . Inactivation of the F4/80 glycoprotein in the mouse germ line.Molecular and cellular biology,22 (22), 8035-8043.

Other Product Names

Ly71, F4/80, Gpf480, TM7LN3, DD7A5-7, EGF-TM7, Emr1

Tested Applications

Flow

Physical Properties

Liquid

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