What is the catalog number for F4/80 Antigen Antibody [BM8.1]?

The catalog number for F4/80 Antigen Antibody [BM8.1] is 76-052.

What sizes are available for F4/80 Antigen Antibody [BM8.1]?

F4/80 Antigen Antibody [BM8.1] is available in the following size(s): 0.1 mg.

Is F4/80 Antigen Antibody [BM8.1] in stock?

F4/80 Antigen Antibody [BM8.1] is currently made to order / not held in immediate stock. Contact us for lead time.

What dilution range is recommended for immunohistochemistry (IHC) with this antibody?

A starting IHC dilution range is listed on the datasheet, but should be optimized for your specific tissue type and staining protocol.

Can I request a Safety Data Sheet (SDS) for this product?

Yes, safety data sheets are available for hazardous or chemically classified products and can be provided on request or downloaded from the product page.

Can this product be used for co-immunoprecipitation (Co-IP)?

Suitability for Co-IP depends on the specific antibody and is noted under validated applications on the datasheet. If it isn’t listed, contact our technical support team for guidance based on your target and sample type.

F4/80 Antigen Antibody [BM8.1]

Product Specifications

Background

The BM8.1 monoclonal antibody specifically binds to the Mouse125 kDa F4/80 antigen, expressed by most mature macrophages. F4/80 is a transmembrane protein used as a marker of macrophages, although it is also expressed on Kupffer and Langerhans cells. The expression of F4/80 antigen is upregulated on bone marrow cells stimulated in vitro with the macrophage colony stimulating factor. The F4/80 antigen is a requirement for the induction of CD8 T cells-mediated peripheral tolerance.

NCBI Gene ID

13733

Swiss Prot

Q61549

Host

Rat

Reactivity

Mouse

Clonality

Monoclonal

Clone

BM8.1

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Innate Immunity

Purification

The monoclonal antibody was purified utilizing affinity chromatography.

Concentration

Batch dependent

Buffer

Phosphate-buffered aqueous solution, ≤0.09% Sodium azide, may contain carrier protein/stabilizer, ph7.2.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

The product should be stored undiluted at 4˚ C . Do not freeze.

Fragment

Rat IgG2a, kappa

Specificity

The BM8.1 monoclonal antibody specifically binds to the Mouse125 kDa F4/80 antigen, expressed by most mature macrophages.

Symbol

Emr1

NCBI Official Name

EGF-like module containing, mucin-like, hormone receptor-like sequence 1

NCBI Organism

Mus musculus

Background Reference 01

Zwadlo, G., Brcker, E. B., Von Bassewitz, D. B., Feige, U., Sorg, C. (1985) . A monoclonal antibody to a differentiation antigen present on mature human macrophages and absent from monocytes.The Journal of Immunology,134 (3), 1487-1492.

Background Reference 02

Leenen, P. J., de Bruijn, M. F., Voerman, J. S., Campbell, P. A., van Ewijk, W. (1994) . Markers of mouse macrophage development detected by monoclonal antibodies.Journal of immunological methods,174 (1), 5-19.

Background Reference 03

Schaller, E., Macfarlane, A. J., Rupec, R. A., Gordon, S., McKnight, A. J., Pfeffer, K. (2002) . Inactivation of the F4/80 glycoprotein in the mouse germ line.Molecular and cellular biology,22 (22), 8035-8043.