CD80 Antibody [16-10A1]

The 16-10A1 antibody reacts with mouse CD80, also known as B7-1, a 55 kDa type I transmembrane protein ligand for CD152 (CTLA-4) and for CD28, a co-stimulatory receptor for the T cell receptor (TCR) . CD28 also binds a second B7 ligand known as CD86 (B7-2) . Both CD80 and CD86 are expressed on activated B cells and antigen-presenting cells. These ligands trigger CD28 signaling in concert with TCR activation to drive T cell proliferation, induce high-level expression of IL-2, impart resistance to apoptosis, and enhance T cell cytotoxicity. The interaction / co-stimulatory signaling between the B7 ligands and CD28 or CTLA-4 provides crucial communication between T cells and B cells or APCs to coordinate the adaptive immune response.

The monoclonal antibody was purified utilizing affinity chromatography. The endotoxin level is determined by LAL test to be less than 0.01 EU/µg of the protein.

The 16-10A1 antibody reacts with mouse CD80, also known as B7-1, a 55 kDa type I transmembrane protein ligand for CD152 (CTLA-4) and for CD28, a co-stimulatory receptor for the T cell receptor (TCR) .

Razi-Wolf, Z., Freeman, G. J., Galvin, F., Benacerraf, B., Nadler, L., Reiser, H. (1992) . Expression and function of the murine B7 antigen, the major costimulatory molecule expressed by peritoneal exudate cells.Proceedings of the National Academy of Sciences,89 (9), 4210-4214.

Hathcock, K. S., Laszlo, G., Pucillo, C., Linsley, P., Hodes, R. J. (1994) . Comparative analysis of B7-1 and B7-2 costimulatory ligands: expression and function.The Journal of experimental medicine,180 (2), 631-640.

Harlan, D. M., Hengartner, H., Huang, M. L., Kang, Y. H., Abe, R., Moreadith, R. W., ... Freeman, G. J. (1994) . Mice expressing both B7-1 and viral glycoprotein on pancreatic beta cells along with glycoprotein-specific transgenic T cells develop diabetes due to a breakdown of T-lymphocyte unresponsiveness.Proceedings of the National Academy of Sciences,91 (8), 3137-3141.