CD40 Antibody [HM40-3]

Product Specifications

Background

The HM40-3 monoclonal antibody reacts with mouse CD40, a 48kDA type I glycoprotein known as Bp50. CD40 is part of the TNFR family and expressed on a subset of T cells, B cells, dendritic cells, and macrophages. It has been shown to be involved in the protection of B cells from apoptosis, B cell differentiation, co-stimulation, and isotype class-switching. The HM40-3 antibody blocks the binding of CD40L (CD154) and stimulates B and dendritic cell proliferation.

NCBI Gene ID

21939

Swiss Prot

P27512

Host

Hamster

Reactivity

Mouse, Rat

Clonality

Monoclonal

Clone

HM40-3

Conjugation

Unconjugated

Type

Primary Antibodies, Functional Antibodies

Field of Research

Immunology

Purification

The monoclonal antibody was purified utilizing affinity chromatography. The endotoxin level is determined by LAL test to be less than 0.01 EU/µg of the protein.

Concentration

Batch dependent

Buffer

Phosphate-buffered aqueous solution, ph7.2.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

The product should be stored undiluted at 4˚ C . Do not freeze.

Fragment

Armenian Hamster IgM, kappa

Specificity

The HM40-3 monoclonal antibody reacts with mouse CD40, a 48kDA type I glycoprotein known as Bp50.

Symbol

Cd40

NCBI Official Name

CD40 antigen

NCBI Organism

Mus musculus

Background Reference 01

Kaneko, Y., Hirose, S., Abe, M., Yagita, H., Okumura, K., Shirai, T. (1996) . CD40‐mediated stimulation of B1 and B2 cells: implication in autoantibody production in murine lupus.European journal of immunology,26 (12), 3061-3065.

Background Reference 02

Ferlin, W. G., Severinson, E., Strm, L., Heath, A. W., Coffman, R. L., Ferrick, D. A., Howard, M. C. (1996) . CD40 signaling induces interleukin‐4‐independent IgE switching in vivo.European journal of immunology,26 (12), 2911-2915.

Background Reference 03

Kashiwada, M., Kaneko, Y., Yagita, H., Okumura, K., Takemori, T. (1996) . Activation of mitogen‐activated protein kinases via CD40 is distinct from that stimulated by surface IgM on B cells.European journal of immunology,26 (7), 1451-1458.