IRS 1 (phospho Ser312) Antibody

Product Specifications

Background

May mediate the control of various cellular processes by insulin. When phosphorylated by the insulin receptor binds specifically to various cellular proteins containing SH2 domains such as phosphatidylinositol 3-kinase p85 subunit or GRB2. Activates phosphatidylinositol 3-kinase when bound to the regulatory p85 subunit

NCBI Gene ID

3667

Swiss Prot

P35568

Accession Number

NP_005535.1

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

IRS-1 (Phospho-Ser312) antibody was raised against a peptide sequence around phosphorylation site of serine 312 (A-T-S (p) -P-A) derived from Human IRS-1.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Phospho-Specific

Purification

Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

Phospho-Specific

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Calculated Molecular Weight

180 kDa

Specificity

This antibody detects endogenous levels of IRS-1 only when phosphorylated at serine 312.

Applications Notes

Western Blot: 1:500~1:1000

Symbol

IRS1

NCBI Official Name

Insulin receptor substrate 1

NCBI Organism

Homo sapiens

Background Reference 01

Tzatsos A, et al. (2006) Mol Cell Biol; 26 (1) : 63-76Ozes ON, et al. (2001) Proc Natl Acad Sci U S A; 98 (8) : 4640-4645Szanto I, et al. (2000) Proc Natl Acad Sci U S A; 97 (5) : 2355-2360Ozes ON, et al. (2001) Proc Natl Acad Sci U S A; 98 (8) : 4640-4645