Vav (phospho Tyr174) Antibody

Product Specifications

Background

Couples tyrosine kinase signals with the activation of the Rho/Rac GTPases, thus leading to cell differentiation and/or proliferation

NCBI Gene ID

7409

Swiss Prot

P15498

Accession Number

NP_005419.2

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Vav (Phospho-Tyr174) antibody was raised against a peptide sequence around phosphorylation site of tyrosine 174 (E-I-Y (p) -E-D) derived from Human Vav.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Phospho-Specific

Purification

Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

Phospho-Specific

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Calculated Molecular Weight

95 kDa

Specificity

This antibody detects endogenous level of Vav only when phosphorylated at tyrosine 174.

Applications Notes

Western Blot: 1:500~1:1000

Symbol

VAV1

NCBI Official Name

Vav 1 guanine nucleotide exchange factor

NCBI Organism

Homo sapiens

Background Reference 01

Amarasinghe GK, et al. (2005) Biochemistry; 44 (46) : 15257-68. Charvet C, et al. (2005) J Biol Chem; 280 (15) : 15289-99. Miura-Shimura Y, et al. (2003) J Biol Chem; 278 (40) : 38495-504. Kuhne MR, et al. (2000) J Biol Chem; 275 (3) : 2185-90.