MyoD (phospho Ser200) Antibody

Product Specifications

Background

MyoD encodes a nuclear protein that belongs to the basic helix-loop-helix family of transcription factors and themyogenic factors subfamily. It regulates muscle cell differentiation by inducing cell cycle arrest, a prerequisite formyogenic initiation. The protein is also involved in muscle regeneration. It activates its own transcription which may stabilize commitment to myogenesis.

NCBI Gene ID

4654

Swiss Prot

P15172

Accession Number

NP_002469.2

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

MyoD (Phospho-Ser200) antibody was raised against a peptide sequence around phosphorylation site of serine 200 (A-S-S (p) -P-R) derived from Human MyoD.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Phospho-Specific

Purification

Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

Phospho-Specific

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Calculated Molecular Weight

40 kDa

Specificity

This antibody detects endogenous level of MyoD only when phosphorylated at serine 200.

Applications Notes

Western Blot: 1:500~1:1000

Symbol

MYOD1

NCBI Official Name

Myogenic differentiation 1

NCBI Organism

Homo sapiens

Background Reference 01

Emmanuel G, et al. J. Biol. Chem., Jun 2000; 275: 18767 - 18776Tintignac LA, et al. Mol Cell Biol. 2004 Feb; 24 (4) : 1809-1821. Kitzmann M, et a. Mol Cell Biol. 1999 Apr; 19 (4) : 3167-3176.