FAK (phospho Tyr861) Antibody

Product Specifications

Background

Non-receptor protein-tyrosine kinase implicated in signaling pathways involved in cell motility, proliferation and apoptosis. Activated by tyrosine-phosphorylation in response to either integrin clustering induced by cell adhesion or antibody cross-linking, or via G-protein coupled receptor (GPCR) occupancy by ligands such as bombesin or lysophosphatidic acid, or via LDL receptor occupancy. Plays a potential role in oncogenic transformations resulting in increased kinase activity.

NCBI Gene ID

5747

Swiss Prot

Q05397

Accession Number

NP_005598.3

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

FAK (Phospho-Tyr861) antibody was raised against a peptide sequence around phosphorylation site of tyrosine 861 (H-I-Y (p) -Q-P) derived from Human FAK.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Phospho-Specific

Purification

Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

Phospho-Specific

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Calculated Molecular Weight

125 kDa

Specificity

This antibody detects endogenous level of FAK only when phosphorylated at tyrosine 861.

Applications Notes

Western Blot: 1:500~1:1000

Symbol

PTK2

NCBI Official Name

Protein tyrosine kinase 2

NCBI Organism

Homo sapiens

Background Reference 01

Shi Q, et al. (2003) Mol Biol Cell; 14 (10) : 4306-15. Vadlamudi RK, et al. (2003) FEBS Lett; 543 (1-3) : 76-80. Eliceiri BP, et al. (2002) J Cell Biol Apr 01; 157 (1) : 149-60. Abu-Ghazaleh R, (2001) et al. Biochem J; 360 (Pt 1) : 255-64.