P95 / NBS1 (phospho Ser343) Antibody

Product Specifications

Background

Mutations in p95/NBS1 gene are associated with Nijmegen breakage syndrome, an autosomal recessive chromosomal instability syndrome characterized by microcephaly, growth retardation, immunodeficiency, and cancer predisposition. The encoded protein is a member of the MRE11/RAD50 double-strand break repair complex which consists of 5 proteins. This gene product is thought to be involved in DNA double-strand break repair and DNA damage-induced checkpoint activation.

NCBI Gene ID

4683

Swiss Prot

O60934

Accession Number

NP_002476.2

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

P95/NBS1 (Phospho-Ser343) antibody was raised against a peptide sequence around phosphorylation site of serine 343 (S-L-S (p) -Q-G) derived from Human p95/NBS1.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Phospho-Specific, Cell Cycle

Purification

Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

Phospho-Specific

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Calculated Molecular Weight

95 kDa

Specificity

This antibody detects endogenous level of p95/NBS1 only when phosphorylated at serine 343.

Applications Notes

Western Blot: 1:500~1:1000

Symbol

NBN

NCBI Official Name

Nibrin

NCBI Organism

Homo sapiens

Background Reference 01

Hsu HL, et al (2005) Oncogene; 24 (31) : 4956-64. Falck J, et al. (2005) Nature; 434 (7033) : 605-11. Buscemi G, et al. (2004) Oncogene; 23 (46) : 7691-700. Beausoleil SA, et al. (2004) Proc Natl Acad Sci U S A; 101 (33) : 12130-5.