HNF4a (phospho Ser304) Antibody

Product Specifications

Background

The protein encoded by this gene is a nuclear transcription factor which binds DNA as a homodimer. The encoded protein controls the expression of several genes, including hepatocyte nuclear factor 1 alpha, a transcription factor which regulates the expression of several hepatic genes. This gene may play a role in development of the liver, kidney, and intestines. Mutations in this gene have been associated with monogenic autosomal dominant non-insulin-dependent diabetes mellitus type I. Alternative splicing of this gene results in multiple transcript variants.

NCBI Gene ID

3172

Swiss Prot

P41235

Accession Number

NP_000448.3

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

HNF4a (Phospho-Ser304) antibody was raised against a peptide sequence around phosphorylation site of serine 304 (L-R-S (p) -Q-V) derived from Human HNF4α .

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Phospho-Specific

Purification

Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

Phospho-Specific

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Calculated Molecular Weight

52 kDa

Specificity

This antibody detects endogenous level of HNF4α only when phosphorylated at serine 304.

Applications Notes

Western Blot: 1:1000

Symbol

HNF4A

NCBI Official Name

Hepatocyte nuclear factor 4, alpha

NCBI Organism

Homo sapiens

Background Reference 01

Kritis A. A, et al. Gene 173:275-280 (1996) Drewes T, et al. Mol. Cell. Biol. 16:925-931 (1996) Yamagata K, et al. Nature 384:458-460 (1996) Tanaka T, et al. Submitted (JUL-2004) to the EMBL/GenBank/DDBJ databases