HDAC4 / HDAC5 / HDAC9 (Ab 246 / 259 / 220) Antibody

Product Specifications

Background

Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4) . Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Involved in muscle maturation via its interaction with the myocyte enhancer factors such as MEF2A, MEF2C and MEF2D.

NCBI Gene ID

9759;10014;9734

Swiss Prot

P56524Q9UQL6Q9UKV0

Accession Number

NP_006028.2; NP_001015053.1; NP_055522.1

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

HDAC4/HDAC5/HDAC9 (Ab-246/259/220) antibody was raised against a peptide sequence around aa.244~248/257~261/218~222 (T-A-S-E-P) derived from Human HDAC4/HDAC5/HDAC9.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Cell Cycle

Purification

Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Calculated Molecular Weight

140, 124 kDa

Specificity

This antibody detects endogenous level of total HDAC4/HDAC5/HDAC9 protein.

Applications Notes

Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200

Symbol

HDAC4 HDAC5 HDAC9

NCBI Official Name

Histone deacetylase 4 / histone deacetylase 5 / histone deacetylase 9

NCBI Organism

Homo sapiens

Background Reference 01

Cress, W. D. and Seto, E. (2000) J Cell Physiol 184, 1-16. Vigushin, D. M. and Coombes, R. C. (2004) Curr. Cancer Drug Targets 4, 205-218. Marmorstein, R. (2001) Cell Mol Life Sci 58, 693-703. Thiagalingam, S. et al. (2003) Ann. N. Y. Acad. Sci. 983, 84-100.