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EIF4B (Ab 422) Antibody

Product Specifications

Background

Required for the binding of mRNA to ribosomes. Functions in close association with EIF4-F and EIF4-A. Binds near the 5'-terminal cap of mRNA in presence of EIF-4F and ATP. Promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both EIF4-A and EIF4-F.

NCBI Gene ID

1975

Swiss Prot

P23588

Accession Number

NP_001408.2

Host

Rabbit

Reactivity

Human

Immunogen

EIF4B (Ab-422) antibody was raised against a peptide sequence around aa.420~424 (T-G-S-E-S) derived from Human eIF4B.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Other

Purification

Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Product Datasheet

https://www.prosci-inc.com/?datasheet_sku=79-421

Calculated Molecular Weight

80 kDa

Specificity

This antibody detects endogenous level of total eIF4B protein.

Applications Notes

Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100

Symbol

EIF4B

NCBI Official Name

Eukaryotic translation initiation factor 4B

NCBI Organism

Homo sapiens

Background Reference 01

Gingras, A. C. et al. (2001) Genes Dev. 15, 807-826. Duncan, R. and Hershey, J. W. (1985) J. Biol. Chem. 260, 5493-5497. Duncan, R. F. and Hershey, J. W. (1989) J. Cell Biol. 109, 1467-1481.

Other Product Names

EIF-4B, PRO1843, PKB, RAC

Tested Applications

WB, IHC

Protein ID

50053795

Physical Properties

Liquid

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