Smad2 (Ab 220) Antibody

Product Specifications

Background

Transcriptional modulator activated by TGF-beta and activin type 1 receptor kinase. SMAD2 is a receptor-regulated SMAD (R-SMAD) . May act as a tumor suppressor in colorectal carcinoma.

NCBI Gene ID

4087

Swiss Prot

Q15796

Accession Number

NP_001003652.1

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Smad2 (Ab-220) antibody was raised against a peptide sequence around aa.218~222 (P-E-T-P-P) derived from Human Smad2.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Other

Purification

Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Calculated Molecular Weight

60 kDa

Specificity

This antibody detects endogenous level of total Smad2 protein.

Applications Notes

Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100

Symbol

SMAD2

NCBI Official Name

SMAD family member 2

NCBI Organism

Homo sapiens

Background Reference 01

Sang Gyun Kim et al. (2004) Mol Biol Cell. February; 15 (2) : 420–434. Rebecca A. Randall et al. (2004) Mol Cell Biol. February; 24 (3) : 1106–1121. Kimberly A Brown et al. (2004) Breast Cancer Res. ; 6 (3) : R215–R231.