Shc1 (Ab 427) Antibody

Product Specifications

Background

Signaling adapter that couples activated growth factor receptors to signaling pathway. Isoform p46Shc and isoform p52Shc, once phosphorylated, couple activated receptor tyrosine kinases to Ras via the recruitment of the GRB2/SOS complex and are implicated in the cytoplasmic propagation of mitogenic signals. Isoform p46Shc and isoform p52Shc may thus function as initiators of the Ras signaling cascade in various non-neuronal systems. Isoform p66Shc does not mediate Ras activation, but is involved in signal transduction pathways that regulate the cellular response to oxidative stress and life span. Isoform p66Shc acts as a downstream target of the tumor suppressor p53 and is indispensable for the ability of stress-activated p53 to induce elevation of intracellular oxidants, cytochrome c release and apoptosis. The expression of isoform p66Shc has been correlated with life span

NCBI Gene ID

6464

Swiss Prot

P29353

Accession Number

NP_001123512.1

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Shc1 (Ab-427) antibody was raised against a peptide sequence around aa.425~429 (P-S-Y-V-N) derived from Human Shc1.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Other

Purification

Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Calculated Molecular Weight

46, 52, 66 kDa

Specificity

This antibody detects endogenous level of total Shc1 protein.

Applications Notes

Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200

Symbol

SHC1

NCBI Official Name

SHC (Src homology 2 domain containing) transforming protein 1

NCBI Organism

Homo sapiens

Background Reference 01

Trampont P, et al. (2006) Mol Cell Biol; 26 (23) : 9035-9044. Patrussi L, et al. (2005) Oncogene; 24 (13) : 2218-2228van der Geer P, et al. (1996) Curr Biol ; 6 (11) : 1435-1444