PKCzeta (Ab 410) Antibody

Product Specifications

Background

This is a calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme. PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters. Subunit of a quaternary complex that plays a central role in epithelial cell polarization.

NCBI Gene ID

5590

Swiss Prot

Q05513

Accession Number

NP_002735.3

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

PKCzeta (Ab-410) antibody was raised against a peptide sequence around aa. 408~412 (T-S-T-F-C) derived from PKCzeta .

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Neuroscience

Purification

Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Calculated Molecular Weight

78 kDa

Specificity

This antibody detects endogenous levels of total PKCzeta protein.

Applications Notes

Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100

Symbol

PRKCZ

NCBI Official Name

Protein kinase C, zeta

NCBI Organism

Homo sapiens

Background Reference 01

Parmentier JH, et al. (2004) BMC Cell Biol; 5: 4. Castrillo A, et al. (2003) Mol Cell Biol; 23 (4) : 1196-1208. Paramio JM, et al. (2001) Mol Cell Biol; 21 (21) : 7449-7459. Braiman L, et al. (2001) Mol Cell Biol; 21 (22) : 7852-7861.