Keratin 8 (Ab 74) Antibody

Product Specifications

Background

Keratin 8 is a member of the type II keratin family clustered on the long arm of chromosome 12. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. The product of this gene typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis.

NCBI Gene ID

3856

Swiss Prot

P05787

Accession Number

NP_002264.1

Host

Rabbit

Reactivity

Human

Immunogen

Keratin 8 (Ab-74) antibody was raised against a peptide sequence around aa.72~76 (L-L-S-P-L) derived from Human Keratin 8 (CK8) .

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Other

Purification

Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Calculated Molecular Weight

55 kDa

Specificity

This antibody detects endogenous level of total Keratin 8 protein.

Applications Notes

Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200

Symbol

KRT8

NCBI Official Name

Keratin 8

NCBI Organism

Homo sapiens

Background Reference 01

Nakamichi I, et al. (2002) Mol Biol Cell; 13 (10) : 3441-3451. Prochasson P, et al. (2002) Nucleic Acids Res; 30 (15) : 3312-3322. Pankov R, et al. (1994) Proc Natl Acad Sci U S A; 91 (3) : 873-877. Thorey IS, et al. (1993) Mol Cell Biol; 13 (11) : 6742-6751.

Other Product Names

K8, KO, CK8, CK-8, CYK8, K2C8, CARD2, HSTF1, CK 8, Cytokeratin endo A, K8

Tested Applications

WB, IHC, IF

Protein ID

4504919

Physical Properties

Liquid

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