NFkB p100 / p52 (Ab 872) Antibody

Product Specifications

Background

NF-κ -B is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological processed such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-κ -B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively.

NCBI Gene ID

4791

Swiss Prot

Q00653

Accession Number

NP_001070961.1

Host

Rabbit

Reactivity

Human, Mouse

Immunogen

NFkB-p100/p52 (Ab-872) antibody was raised against a peptide sequence around aa.870~874 (S-Q-S-V-E) derived from Human NFκ B-p100/p52.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Apoptosis, Cancer

Purification

Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Calculated Molecular Weight

52, 120 kDa

Specificity

This antibody detects endogenous level of total NFκ B-p100/p52 protein.

Applications Notes

Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200

Symbol

NFKB2

NCBI Official Name

Nuclear factor of kappa light polypeptide gene enhancer in B-cells 2 (p49/p100)

NCBI Organism

Homo sapiens

Background Reference 01

Shin HM, et al. (2006) EMBO J; 25 (1) : 129-138. Li Q, et al. (2005) Proc Natl Acad Sci USA; 102 (35) : 12425-12430. Chen C, et al. (2000) Mol Cell Biol; 20 (8) : 2687-2695.