PKCdelta (Ab 645) Antibody

Product Specifications

Background

This is calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme. PKC is activated by diacylglycerol. which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters. May play a role in antigen-dependent control of B-cell function. Phosphorylates MUC1 in the C-terminal and regulates the interaction between MUC1 and beta-catenin.

NCBI Gene ID

5580

Swiss Prot

Q05655

Accession Number

NP_006245.2

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

PKCdelta (Ab-645) antibody was raised against a peptide sequence around aa. 643~647 (R-L-S-Y-S) derived from Human PKCδ .

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Apoptosis, Cancer, Neuroscience

Purification

Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Calculated Molecular Weight

78 kDa

Specificity

This antibody detects endogenous level of total PKCδ protein.

Applications Notes

Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200

Symbol

PRKCD

NCBI Official Name

Protein kinase C, delta

NCBI Organism

Homo sapiens

Background Reference 01

Kei Sakamoto, et, al. (2003) Am J Physiol Endocrinol Metab, ; 285: E1081 - E1088. Ling Zhang, et, al. (2004) J. Biol. Chem ; 279: 28315 - 28319. Kristof Van Kolen et, al. (2006) FEBS J ; 273: 1843 - 1854. Martin Villalba, et, al. (2002) J. Cell Biol ; 157: 253.