What is the catalog number for P70 S6 Kinase (Ab 424) Antibody?

The catalog number for P70 S6 Kinase (Ab 424) Antibody is 79-397.

What sizes are available for P70 S6 Kinase (Ab 424) Antibody?

P70 S6 Kinase (Ab 424) Antibody is available in the following size(s): 0.1 mg.

Is P70 S6 Kinase (Ab 424) Antibody in stock?

P70 S6 Kinase (Ab 424) Antibody is currently made to order / not held in immediate stock. Contact us for lead time.

Can this product be used for co-immunoprecipitation (Co-IP)?

Suitability for Co-IP depends on the specific antibody and is noted under validated applications on the datasheet. If it isn’t listed, contact our technical support team for guidance based on your target and sample type.

Is this kit compatible with automated liquid handling platforms?

Automation compatibility varies by kit and isn’t always listed on the datasheet. Contact our technical support team with details of your platform so we can confirm with the manufacturer.

What applications has this product been validated for?

Validated applications such as WB, ELISA, IHC, ICC, or FC are listed on the product datasheet. If you need application-specific validation data before ordering, contact our technical support team with the catalogue number.

P70 S6 Kinase (Ab 424) Antibody

Product Specifications

Background

RPS6KB1 phosphorylates the Ribosomal Protein-S6. Activation of RPS6KB1 requires a complex, ordered series of conformational changes and phosphorylation reactions. While the role of sequential, multi-site phosphorylation has been extensively detailed, characterization of the priming step required to initiate this cascade has remained elusive. Probably this priming process is dependent on calcium. Calcium-dependent regulation of RPS6KB1 does not specifically target Thr-229 and Thr-389, the key regulatory phosphorylation sites; rather, calcium chelation results in a global inhibition of RPS6KB1 phosphorylation. The initial calcium-dependent process is required to release an inhibitory interaction between the C- and N-termini of RPS6KB1, thus allowing phosphorylation of key domains. The priming event involves formation of a calcium-dependent protein complex that releases the interaction between the N- and C-termini. RPS6KB1 is then accessible for activation by the kinases that target the known regulatory phosphorylation sites .

NCBI Gene ID

6198

Swiss Prot

P23443

Accession Number

NP_003152.1

Host

Rabbit

Reactivity

Human

Immunogen

P70 S6 Kinase (Ab-424) antibody was raised against a peptide sequence around aa.422~426 (P-V-S-P-V) derived from Human p70S6k.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Apoptosis, Cancer

Purification

Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Calculated Molecular Weight

70, 85 kDa

Specificity

This antibody detects endogenous level of total p70 S6 Kinase protein.

Applications Notes

Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200

Symbol

RPS6KB1

NCBI Official Name

Ribosomal protein S6 kinase, 70kDa, polypeptide 1

NCBI Organism

Homo sapiens

Background Reference 01

Satoru Eguchi et al. (1999) J Biol Chem, Vol. 274: 36843-36851Papst PJ, et al. (1998) J Biol Chem. 273 (24) : 15077-84. Ulrike Krause et al. (2002) Eur. J. Biochem. 269: 3751-3759 cLe, X. F, et al. (2003) Oncogene 22: 484–97