Connexin 43 (Ab 367) Antibody

Product Specifications

Background

One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell. May play a critical role in the physiology of hearing by participating in the recycling of potassium to the cochlear endolymph.

NCBI Gene ID

2697

Swiss Prot

P17302

Accession Number

NP_000156.1

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Connexin 43 (Ab-367) antibody was raised against a peptide sequence around aa. 365~369 (R-A-S-S-R) derived from Human Connexin 43.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Other

Purification

Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Calculated Molecular Weight

43 kDa

Specificity

This antibody detects endogenous level of total Connexin43 protein.

Applications Notes

Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100

Symbol

GJA1

NCBI Official Name

Gap junction protein, alpha 1, 43kDa

NCBI Organism

Homo sapiens

Background Reference 01

Joell L. Solan1, et al. (2003) Cell Science 116: 2203-2211Satoshi Matsushita, et al. (2006) Histochemistry and Cytochemistry 54 (3) : 343-353, Xiaoyong Bao, et al. (2004) Cell Physiol 286: C647-C654W. E. I. Li, et al. (1998) European Journal of Neuroscience 10: 2444