HSF1 (Ab 307) Antibody

Product Specifications

Background

DNA-binding protein that specifically binds heat shock promoter elements (HSE) and activates transcription. In higher eukaryotes, HSF is unable to bind to the HSE unless the cells are heat shocked.

NCBI Gene ID

3297

Swiss Prot

Q00613

Accession Number

NP_005517.1

Host

Rabbit

Reactivity

Human

Immunogen

HSF1 (Ab-307) antibody was raised against a peptide sequence around aa. 305~309 (P-Q-S-P-R) derived from Human HSF1.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Other

Purification

Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Calculated Molecular Weight

82 kDa

Specificity

This antibody detects endogenous level of total HSF1 protein.

Applications Notes

Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100

Symbol

HSF1

NCBI Official Name

Heat shock transcription factor 1

NCBI Organism

Homo sapiens

Background Reference 01

Guettouche T, et al. (2005) BMC Biochem ; 6 (1) : 4Wang X, et al. (2003) Mol Cell Biol ; 23 (17) : 6013-6026Jolly C, et al. (1999) Proc Natl Acad Sci USA; 96 (12) : 6769-6774.