Paxillin (Ab 31) Antibody

Product Specifications

Background

An antiphosphotyrosine antibody was used to identify proteins that are phosphorylated in Rous sarcoma virus-transformed chick embryo fibroblasts, and a 76-kD protein was obtained that localizes to focal adhesions at the ends of actin-containing stress fibers in nontransformed cells (Ref.1) . This protein was purified from chicken gizzard smooth muscle, and was named Pxn (Paxillin) ('paxillus' means 'small stake' or 'peg' in Latin) as a protein tethered to the membrane at focal adhesions

NCBI Gene ID

5829

Swiss Prot

P49023

Accession Number

NP_001074324.1

Host

Rabbit

Reactivity

Human, Rat

Immunogen

Paxillin (Ab-31) antibody was raised against a peptide sequence around aa.29~33 (T-P-Y-S-Y) derived from Human Paxillin.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Other

Purification

Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Calculated Molecular Weight

68 kDa

Specificity

This antibody detects endogenous level of total Paxillin protein.

Applications Notes

Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100

Symbol

PXN

NCBI Official Name

Paxillin

NCBI Organism

Homo sapiens

Background Reference 01

Davidson D, et al. (2001) EMBO J; 20 (13) : 3414-3426Kook S, et al. (2000) Mol Biol Cell; 11 (3) : 929-939Fleming I, et al. (1999) Proc Natl Acad Sci U S A; 96 (3) : 1123-1128Goldberg MB, et al. (2001) Microbiol Mol Biol Rev; 65 (4) : 595-626Thomas JT, et al. (1999) Proc Natl Acad Sci U S A; 96 (15) : 8449-8454