What is the catalog number for AMPKa1 / AMPKa2 (Ab 174 / 172) Antibody?

The catalog number for AMPKa1 / AMPKa2 (Ab 174 / 172) Antibody is 79-357.

What sizes are available for AMPKa1 / AMPKa2 (Ab 174 / 172) Antibody?

AMPKa1 / AMPKa2 (Ab 174 / 172) Antibody is available in the following size(s): 0.1 mg.

Is AMPKa1 / AMPKa2 (Ab 174 / 172) Antibody in stock?

AMPKa1 / AMPKa2 (Ab 174 / 172) Antibody is currently made to order / not held in immediate stock. Contact us for lead time.

Do you offer custom or private-label production of this type of product?

Yes, custom antibody development and private-label production services are available. Contact our sales team to discuss your target, timeline, and quantity requirements.

What is the immunogen used to generate this antibody?

Immunogen or antigen details used to raise this antibody are listed on the product datasheet under immunogen information.

What is the recommended positive control for this assay?

A recommended positive control, where one is specified by the manufacturer, is listed on the datasheet or in the assay protocol.

AMPKa1 / AMPKa2 (Ab 174 / 172) Antibody

Product Specifications

Background

Responsible for the regulation of fatty acid synthesis by phosphorylation of acetyl-CoA carboxylase. It also regulates cholesterol synthesis via phosphorylation and inactivation of hormone-sensitive lipase and hydroxymethylglutaryl-CoA reductase. Appears to act as a metabolic stress-sensing protein kinase switching off biosynthetic pathways when cellular ATP levels are depleted and when 5'-AMP rises in response to fuel limitation and/or hypoxia. This is a catalytic subunit.

NCBI Gene ID

5562;5563

Swiss Prot

Q13131

Accession Number

NP_006242.5; NP_006243.2

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

AMPKa1/AMPKa2 (Ab-174/172) antibody was raised against a peptide sequence around aa. 172~176/170~174 (L-R-T-S-C) derived from Human AMPKα1/AMPKα2.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Other

Purification

Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Calculated Molecular Weight

63 kDa

Specificity

This antibody detects endogenous level of total AMPKα1/AMPKα2 protein.

Applications Notes

Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200

Symbol

PRKAA1 PRKAA2

NCBI Official Name

Protein kinase, AMP-activated, alpha 1 catalytic subunit / protein kinase, AMP-activated, alpha 2 catalytic subunit

NCBI Organism

Homo sapiens

Background Reference 01

Hurley RL, et al. (2005) J Biol Chem. Aug 12; 280 (32) : 29060-29066Woods A, et al. (2003) Curr Biol. Nov 11; 13 (22) : 2004-2008Nielsen JN, et al. (2003) J Appl Physiol. Feb; 94 (2) : 631-641Da Silva Xavier G, et al. (2000) Proc Natl Acad Sci U S A. Apr 11; 97 (8) : 4023-4028.